Reverse Genetic Application in functional study of Arabidopsis thaliana strictosidine synthase Like-gene under salt stress

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Pourkarimi Daryakenari Mohadece; SOHANI MOHAMMAD MEHDI; ABEDI AMIN

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Paper Information

Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2018 | Volume:7 | Issue:1 Page(s): 21-32

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Information Journal Paper

Title

Reverse Genetic Application in functional study of Arabidopsis thaliana strictosidine synthase Like-gene under salt stress

Author(s)

Pourkarimi Daryakenari Mohadece | SOHANI MOHAMMAD MEHDI | ABEDI AMIN | Issue Writer Certificate

Keywords

Gene Expression

Negatively Regulator

Loss of Function Gene

T-DNA Line

Abstract

Strictosidine synthase-like (SSL) is a group of gene family in the Arabidopsis thaliana genome, which their orthologous in other plants, such as Catharanthus roseus, are key enzymes in the monoterpenoid indole alkaloid biosynthesis pathway. Arabidopsis SSL gene, a member of SSL family, has been induced significantly by various stresses and signaling molecules. In this study, the reverse genetics approaches (T-DNA insertion) are used to determine the function of the SSL gene in response to the salt stress. The experiment was conducted using a factorial experiment based on the completely randomized design with two factors and three replications. These factors were included two genotypes (Col-and ssl ) and three NaCl treatments ( mM, mM and mM). Compared with the wild-type, the ssl mutant shows significantly increased proline accumulation, anthocyanin content and reduced malondialdehyde (MDA) content in response to the salt stress. Real-Time PCR analysis revealed that the expression levels of SOS genes were upregulated significantly in ssl compared with the expression in Col-in response to mM NaCl at h and h after the treatment. Totally, these results suggest that SSL might have a negative regulatory role in response to salt stress in Arabidopsis.

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APA: Copy

Pourkarimi Daryakenari, Mohadece, SOHANI, MOHAMMAD MEHDI, & ABEDI, AMIN. (2018). Reverse Genetic Application in functional study of Arabidopsis thaliana strictosidine synthase Like-gene under salt stress. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 7(1 ), 21-32. SID. https://sid.ir/paper/270540/en

Vancouver: Copy

Pourkarimi Daryakenari Mohadece, SOHANI MOHAMMAD MEHDI, ABEDI AMIN. Reverse Genetic Application in functional study of Arabidopsis thaliana strictosidine synthase Like-gene under salt stress. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2018;7(1 ):21-32. Available from: https://sid.ir/paper/270540/en

IEEE: Copy

Mohadece Pourkarimi Daryakenari, MOHAMMAD MEHDI SOHANI, and AMIN ABEDI, “Reverse Genetic Application in functional study of Arabidopsis thaliana strictosidine synthase Like-gene under salt stress,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 7, no. 1 , pp. 21–32, 2018, [Online]. Available: https://sid.ir/paper/270540/en

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