Grapevine fanleaf virus 2AHP gene shows more diversity at amino acid level rather than nucleotide level

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Enteshari Marziyeh; Nourinejhad Zarghani Shaheen; NASSAJ HOSSEINI SAYED MOHSEN

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Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2018 | Volume:7 | Issue:1 Page(s): 79-90

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Title

Grapevine fanleaf virus 2AHP gene shows more diversity at amino acid level rather than nucleotide level

Author(s)

Enteshari Marziyeh | Nourinejhad Zarghani Shaheen | NASSAJ HOSSEINI SAYED MOHSEN | Issue Writer Certificate

Keywords

Grapevine

GFLV

sequencing

phylogeny

nepoviruses

2AHP

Abstract

Grapevine fanleaf virus (GFLV) has been reported from vineyards worldwide. The virus causes different symptoms categorized as three distinct syndromes including fan leaf degeneration, yellow mosaic, and vein banding. These variations in the symptoms can be addressed by analysis of genetic diversity of the virus. The aim of the present study was to estimate genetic diversity of the corresponding 2AHP gene in GFLV isolates especially the ones that are associated with the yellow mosaic syndrome. Accordingly, the Grapevine samples were collected from vineyards in Bonab, Shir-Amin, Hossein-Abad, and Tabriz in East Azarbaijan Province of Iran. After amplification of GFLV 2AHP gene from the infected samples via RTPCR, the products were cloned and sequenced. The sequenced GFLV 2AHP gene from 11 different isolates showed 0. 8-27% and 0. 8-35 % diversity at nucleotide and amino acid levels, respectively, denoting a higher diversity of 2AHP amino acid sequence than its nucleotide sequence. Likewise, presence of a hot spot for recombination events on 2AHP region was explored by the use of recombination analysis. Moreover, it was found that the selection pressure on 2AHP region is not uniformly distributed so that the ratio (dn/ds) for the N-terminus proximity, between the nucleotides 408-564, was more than one. Therefore, synonymous point mutations as well as recombination events might be reason for this evidence.

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APA: Copy

Enteshari, Marziyeh, Nourinejhad Zarghani, Shaheen, & NASSAJ HOSSEINI, SAYED MOHSEN. (2018). Grapevine fanleaf virus 2AHP gene shows more diversity at amino acid level rather than nucleotide level. GENETIC ENGINEERING AND BIOSAFETY JOURNAL, 7(1 ), 79-90. SID. https://sid.ir/paper/270545/en

Vancouver: Copy

Enteshari Marziyeh, Nourinejhad Zarghani Shaheen, NASSAJ HOSSEINI SAYED MOHSEN. Grapevine fanleaf virus 2AHP gene shows more diversity at amino acid level rather than nucleotide level. GENETIC ENGINEERING AND BIOSAFETY JOURNAL[Internet]. 2018;7(1 ):79-90. Available from: https://sid.ir/paper/270545/en

IEEE: Copy

Marziyeh Enteshari, Shaheen Nourinejhad Zarghani, and SAYED MOHSEN NASSAJ HOSSEINI, “Grapevine fanleaf virus 2AHP gene shows more diversity at amino acid level rather than nucleotide level,” GENETIC ENGINEERING AND BIOSAFETY JOURNAL, vol. 7, no. 1 , pp. 79–90, 2018, [Online]. Available: https://sid.ir/paper/270545/en

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