Leaf proteome analysis of maize hybrid SC704 under salinity stress

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Aliyari Rad Shiva; BANDEHAGH ALI; DEZHSETAN SARA; BEHNAMIAN MAHDI; MOHARRAMNEJAD SAJJAD

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Journal Paper

Paper Information

Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2019 | Volume:8 | Issue:1 Page(s): 39-50

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Title

Leaf proteome analysis of maize hybrid SC704 under salinity stress

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Keywords

Abiotic stress

Proteomics

Two-dimensional electrophoresis

Abstract

Salinity stress is one of the most important Abiotic stresses that can reduce the yield of crops such as maize. In order to evaluate the effect of salinity stress on maize hybrid SC704 an experiment was performed in completely randomized design with three replications under hydroponic cultural system. The applied salt stress (0 and 200 mM NaCl) decreed dry weight. leaf proteins were analyzed on two-dimensional gel electrophoresis. Comparison of leaf proteins showed that abundance of 16 protein spots was significantly altered under salinity stress. Identification of these spots showed that these proteins were divided into five functional groups: metabolism and energy production, translation and processing, defense, nitrogen cycle, and other protein-rich changes. V-type proton ATPase subunit F protein decreased significantly and EGG APPARATUS-1 protein had the most abundance, which can play an important role in maintaining plant fertility under stress at reproductive stage and crop production.

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