ENHANCEMENT OF TROPANE ALKALOID PRODUCTION AMONG SEVERAL CLONES AND EXPLANTS TYPES OF HAIRY ROOT OF ATROPA BELLADONNA L.

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ESKANDARI SAMET ALI; PIRI KHOSRO; KAYHANFAR MEHRNAZ; HASANLOO TAHEREH

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Journal Paper

Paper Information

Journal: JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS Year:2012 | Volume:1 | Issue:1 Page(s): 35-42

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Information Journal Paper

Title

ENHANCEMENT OF TROPANE ALKALOID PRODUCTION AMONG SEVERAL CLONES AND EXPLANTS TYPES OF HAIRY ROOT OF ATROPA BELLADONNA L.

Author(s)

ESKANDARI SAMET ALI | PIRI KHOSRO | KAYHANFAR MEHRNAZ | HASANLOO TAHEREH | Issue Writer Certificate

Keywords

HAIRY ROOT

BACTERIAL STRAIN

EXPLANTS TYPE

TROPANE ALKALOID

Abstract

Agrobacterium rhizogenes (pRi), a causative agent of HAIRY ROOT disease, effectively induces HAIRY ROOT formation in a variety of plant species. In our study four BACTERIAL STRAINs AR15834, A4, 9435 and C318 and three EXPLANTS TYPEs leaf, stems and roots, were examined. HAIRY ROOTs were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by using strain AR15834. The transgenic status of HAIRY ROOTs was confirmed by polymerase chain reaction using rol B specific primers for the presence of rol B gene in the genomes of transformed roots. Six clones of HAIRY ROOTs were established that differed in their morphology. The detacidni stluser taht 4–11 fold increasing in root biomass after 28 days compared with non-transformed seedling roots. The concentration of TROPANE ALKALOIDs hyoscyamine and scopolamine obtained in transformed roots (4 mg/g dry weight) was 3-11 folds more than in non-transformed cultured roots (0.36 mg/g dry weight).

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APA: Copy

ESKANDARI SAMET, ALI, PIRI, KHOSRO, KAYHANFAR, MEHRNAZ, & HASANLOO, TAHEREH. (2012). ENHANCEMENT OF TROPANE ALKALOID PRODUCTION AMONG SEVERAL CLONES AND EXPLANTS TYPES OF HAIRY ROOT OF ATROPA BELLADONNA L.. JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS, 1(1), 35-42. SID. https://sid.ir/paper/331407/en

Vancouver: Copy

ESKANDARI SAMET ALI, PIRI KHOSRO, KAYHANFAR MEHRNAZ, HASANLOO TAHEREH. ENHANCEMENT OF TROPANE ALKALOID PRODUCTION AMONG SEVERAL CLONES AND EXPLANTS TYPES OF HAIRY ROOT OF ATROPA BELLADONNA L.. JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS[Internet]. 2012;1(1):35-42. Available from: https://sid.ir/paper/331407/en

IEEE: Copy

ALI ESKANDARI SAMET, KHOSRO PIRI, MEHRNAZ KAYHANFAR, and TAHEREH HASANLOO, “ENHANCEMENT OF TROPANE ALKALOID PRODUCTION AMONG SEVERAL CLONES AND EXPLANTS TYPES OF HAIRY ROOT OF ATROPA BELLADONNA L.,” JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS, vol. 1, no. 1, pp. 35–42, 2012, [Online]. Available: https://sid.ir/paper/331407/en

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