IN VITRO CALLUS INDUCTION AND ANALYSIS OF ESSENTIAL OIL CONSTITUENTS IN LEAVES AND CALLUS OFPISTACIA ATLANTICA SUBSP. KURDICA

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AGHAEI PEYMAN; BAHRAMNEJAD BAHMAN; MOZAFARI ALI AKBAR

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Journal: JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS Year:2014 | Volume:3 | Issue:1 Page(s): 69-77

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Title

IN VITRO CALLUS INDUCTION AND ANALYSIS OF ESSENTIAL OIL CONSTITUENTS IN LEAVES AND CALLUS OFPISTACIA ATLANTICA SUBSP. KURDICA

Author(s)

AGHAEI PEYMAN | BAHRAMNEJAD BAHMAN | MOZAFARI ALI AKBAR | Issue Writer Certificate

Keywords

CALLUS

PISTACIA ATLANTICA DESF. SUBSP. KURDICA RECH. F.

GC-MS

ANTHOCYANIN

PHENOLIC COMPOUND

Abstract

In order to optimize CALLUS induction in PISTACIA ATLANTICA DESF. SUBSP. KURDICA RECH. F., the effect of different plant growth regulator (PGR) combinations were studied. Inflorescence axis explants were cultured on WPM medium supplemented with 1, 2 and 3 mg/L of TDZ or 1, 2 and 3 mg/L of BA, in combination with 1mg/l different auxins: 2, 4-D, NAA and IBA. CALLUS initiation was observed in all media evaluated and the highest percentage (100%) was observed in medium containing 1 mg/L BA or TDZ and 1 mg/L NAA. Additionally, ANTHOCYANIN, total PHENOLIC COMPOUND of CALLUS and immature fruits were measured. ANTHOCYANIN content of CALLUS and immature fruits were 79.42 ± 4.63 and 61.71 ± 3.81 mg cyanidin-3-glucoside/g fresh weights, respectively. Total phenolic content of CALLUS and fruits were 4.91 ± 0.492 and 5.1 ± 0.780 mg gallic acid/g fresh weights. GC-MS analysis of essential oil constituents ofP. atlantica subs p. kurdica CALLUS and leaves showed 8 kinds of substances in CALLUS and 13 in leaves. Main composition of CALLUS extract were alpha pinene (89.19%), camphene (1.09%) and Bicyclo [3.1.1] heptane, 6, 6-dimethyl-2-methylene (3.40%) and it was alpha Pinene (15.63%), gamma elemene (33.84%) and caryophyllene (9.26%) in leaves.

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APA: Copy

AGHAEI, PEYMAN, BAHRAMNEJAD, BAHMAN, & MOZAFARI, ALI AKBAR. (2014). IN VITRO CALLUS INDUCTION AND ANALYSIS OF ESSENTIAL OIL CONSTITUENTS IN LEAVES AND CALLUS OFPISTACIA ATLANTICA SUBSP. KURDICA. JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS, 3(1), 69-77. SID. https://sid.ir/paper/331466/en

Vancouver: Copy

AGHAEI PEYMAN, BAHRAMNEJAD BAHMAN, MOZAFARI ALI AKBAR. IN VITRO CALLUS INDUCTION AND ANALYSIS OF ESSENTIAL OIL CONSTITUENTS IN LEAVES AND CALLUS OFPISTACIA ATLANTICA SUBSP. KURDICA. JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS[Internet]. 2014;3(1):69-77. Available from: https://sid.ir/paper/331466/en

IEEE: Copy

PEYMAN AGHAEI, BAHMAN BAHRAMNEJAD, and ALI AKBAR MOZAFARI, “IN VITRO CALLUS INDUCTION AND ANALYSIS OF ESSENTIAL OIL CONSTITUENTS IN LEAVES AND CALLUS OFPISTACIA ATLANTICA SUBSP. KURDICA,” JOURNAL OF MEDICINAL PLANTS AND BY PRODUCTS, vol. 3, no. 1, pp. 69–77, 2014, [Online]. Available: https://sid.ir/paper/331466/en

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