مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

EFFECTS OF VENTILATION AND SUCROSE CONCENTRATIONS ON THE GROWTH AND PLANTLET ANATOMY OF MICROPROPAGATED PERSIAN WALNUT PLANTS

Pages

  111-120

Abstract

 Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. Photoautotrophic and PHOTOMIXOTROPHIC systems could diminish these problems. The purpose of this study was to increase the chlorophyll content and photosynthetic ability of shoots grown in vitro and to improve the adaptation of walnut plantlets. Walnut apical leaf buds were cultured in vessels containing DKW medium supplemented with 0, 15, 30 and 45 g L-1 of sucrose. The vessels were closed with a clear polypropylene lid with two syringe filters on the lid (V1) or with a 50 mm microporous polypropylene membrane (V2). Natural ventilation had a significant effect on most of the growth indices. Furthermore, different levels of sucrose had significant effects on growth characteristics. Natural ventilation increased chlorophyll content significantly. By using ventilated vessels containing g L-1 15 sucrose, the total chlorophyll was significantly increased. Stomata under non-ventilated conditions were spherical with wide openings, whereas those in ventilated vessels were elliptical with narrow openings. As a result, ventilated vessels with 15 g L-1of sucrose produced healthy plantlets.

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    APA: Copy

    HASSANKHAH, AMIN, VAHDATI, KOUROSH, LOTFI, MAHMOUD, MIRMASOUMI, MASOUD, PREECE, JOHN, & ASSAREH, MOHAMMAD HASAN. (2014). EFFECTS OF VENTILATION AND SUCROSE CONCENTRATIONS ON THE GROWTH AND PLANTLET ANATOMY OF MICROPROPAGATED PERSIAN WALNUT PLANTS. INTERNATIONAL JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 1(2), 111-120. SID. https://sid.ir/paper/347862/en

    Vancouver: Copy

    HASSANKHAH AMIN, VAHDATI KOUROSH, LOTFI MAHMOUD, MIRMASOUMI MASOUD, PREECE JOHN, ASSAREH MOHAMMAD HASAN. EFFECTS OF VENTILATION AND SUCROSE CONCENTRATIONS ON THE GROWTH AND PLANTLET ANATOMY OF MICROPROPAGATED PERSIAN WALNUT PLANTS. INTERNATIONAL JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2014;1(2):111-120. Available from: https://sid.ir/paper/347862/en

    IEEE: Copy

    AMIN HASSANKHAH, KOUROSH VAHDATI, MAHMOUD LOTFI, MASOUD MIRMASOUMI, JOHN PREECE, and MOHAMMAD HASAN ASSAREH, “EFFECTS OF VENTILATION AND SUCROSE CONCENTRATIONS ON THE GROWTH AND PLANTLET ANATOMY OF MICROPROPAGATED PERSIAN WALNUT PLANTS,” INTERNATIONAL JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 1, no. 2, pp. 111–120, 2014, [Online]. Available: https://sid.ir/paper/347862/en

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