EVALUATE THE FREQUENCY OF FOOT AND MOUTH DISEASE VIRAL CARRIERS IN SLAUGHTERED SHEEP IN MASHHAD INDUSTRIAL ABATTOIR USING RT-PCR

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ZIBAEE S.; REZAEE S.; RASHTIBAF M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Paper Information

Journal: Journal of Veterinary Research Year:2015 | Volume:70 | Issue:1 Page(s): 7-14

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Title

EVALUATE THE FREQUENCY OF FOOT AND MOUTH DISEASE VIRAL CARRIERS IN SLAUGHTERED SHEEP IN MASHHAD INDUSTRIAL ABATTOIR USING RT-PCR

Author(s)

ZIBAEE S. | REZAEE S. | RASHTIBAF M. | Issue Writer Certificate

Keywords

FMD VIRUSQ2

MOLECULAR DETECTIONQ1

SHEEP CARRIERSQ2

Abstract

BACKGROUND: Foot-and-mouth disease (FMD) is endemic in Iran. Molecular techniques for diagnosis of persistent infection or carrier animals have shown a potential ability to improve the detection of a low genome copy number in samples.OBJECTIVES: The purpose of this study is to evaluate the frequency of foot and mouth disease viral carriers in slaughtered sheep in Mashhad industrial abattoir using RT-PCR.METHODS: Samples were isolated from tonsil of 94 slaughtered sheep analyzed by RT-PCR experiment for the detection of FMD, identification of FMD VIRUS serotypes and at the end nucleic acid sequencing were performed.RESULTS: The results showed that the 23 samples (24.5 percent) were positive for the presence of FMD VIRUS RNA, of which 89.9% of cases are type O and 3 cases of FMD samples did not respond. The results of the 1D genome sequencing of the nucleic acid virus showed that FMD VIRUS of sheep (O/IRN/100/2010Sheep), has 92.02% similarity with the virus (O/IRN/67/2001-2005) and 88.42% similarity with the virus (O/IRN/15/2004-2008).CONCLUSIONS: This study showed that the percentage of FMDV SHEEP CARRIERS in Mashhad slaughterhouse was remarkable. Estimation of the frequency of carrier state in cattle and small ruminants is recommended as a monitor of control plan in the region.

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APA: Copy

ZIBAEE, S., REZAEE, S., & RASHTIBAF, M.. (2015). EVALUATE THE FREQUENCY OF FOOT AND MOUTH DISEASE VIRAL CARRIERS IN SLAUGHTERED SHEEP IN MASHHAD INDUSTRIAL ABATTOIR USING RT-PCR. JOURNAL OF VETERINARY RESEARCH, 70(1), 7-14. SID. https://sid.ir/paper/35192/en

Vancouver: Copy

ZIBAEE S., REZAEE S., RASHTIBAF M.. EVALUATE THE FREQUENCY OF FOOT AND MOUTH DISEASE VIRAL CARRIERS IN SLAUGHTERED SHEEP IN MASHHAD INDUSTRIAL ABATTOIR USING RT-PCR. JOURNAL OF VETERINARY RESEARCH[Internet]. 2015;70(1):7-14. Available from: https://sid.ir/paper/35192/en

IEEE: Copy

S. ZIBAEE, S. REZAEE, and M. RASHTIBAF, “EVALUATE THE FREQUENCY OF FOOT AND MOUTH DISEASE VIRAL CARRIERS IN SLAUGHTERED SHEEP IN MASHHAD INDUSTRIAL ABATTOIR USING RT-PCR,” JOURNAL OF VETERINARY RESEARCH, vol. 70, no. 1, pp. 7–14, 2015, [Online]. Available: https://sid.ir/paper/35192/en

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