DETERMINATION OF THE LOWEST VALNABLE TITER (CUT-OFF TITER) OF ANTITOXOPLASMA ANTIBODIES FOR DETECTION OF TOXOPLASMOSIS IN SHEEP BY ELISA AND IFA TESTS

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MORSHEDI A.; MAHMOUDIAN A.; AWAZ A.

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Journal: Journal of Veterinary Research Year:2005 | Volume:60 | Issue:3 Page(s): 219-223

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Title

DETERMINATION OF THE LOWEST VALNABLE TITER (CUT-OFF TITER) OF ANTITOXOPLASMA ANTIBODIES FOR DETECTION OF TOXOPLASMOSIS IN SHEEP BY ELISA AND IFA TESTS

Author(s)

MORSHEDI A. | MAHMOUDIAN A. | AWAZ A. | Issue Writer Certificate

Keywords

TOXOPLASMA GONDIIQ3

IPATQ4

IGG-ELISAQ2

Abstract

Objective: To investigate 1) the cut-off titers of antitoxoplasma antibodies through in IgG- ELISA and IFA tests and 2) application of IgG- ELISA rather than IPA test for diagnosis of toxoplasmosis (TOXOPLASMA GONDII) in mature sheep.Animals: One hundred and ten Iranian mature sheep.Procedure: The serum samples were examined to determine the final positive dilution of antitoxoplasma antibody by IgGELISA and IFA tests. For this purpose, immunoreactivity of the final positive dilution of each serum with to SAG-1 antigen was determined by western blot analysis. The ELlSA microplate coated with 105 tachyzoites/ well, was incubated with diluttd sheep sera. After adding of substrate, enzymatic activity was measured at 492 nm. Samples with higher absorbance values (=2.5 times greater than these values of negative controls) designated as positive. The data obtained from 110 sera with IPA and ELISA was compared with each other to determine the percentage of agreement between them.Statistical analysis: Using a chi-square test to show the significant differences between results of IFA and ELISA. Furthermore, these results were compared to determine the percentage of agreement between them. Results: 24 out of 110 serum samples were concordant, positive (21.8%) and 79 cases (70.9%) were concordant, negative in both IFA and ELISA. All positive samples exhibited strong reactivity to SAG-1 (30 KD band). Among 110 serum samples, five discordant ELISA- positive and two, discordant IFA- positive sera had no reactivity with SAG-I band in WB. Moreover, there is 92.7% coincidence between results of ELISA and IPA tests.Conclusion: In this study the lowest valuable titer, for detection of Toxoplasmosis in sheep, was obtained as 1:40 and 1:100 in the IFA and ELISA tests, respectively.Furthermore, immunoreactivity of serum dilutions to SAG lantigen in the WB proved to be a useful tool for setting of optimum cut-off titers in the IFA and ELISA tests.

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APA: Copy

MORSHEDI, A., MAHMOUDIAN, A., & AWAZ, A.. (2005). DETERMINATION OF THE LOWEST VALNABLE TITER (CUT-OFF TITER) OF ANTITOXOPLASMA ANTIBODIES FOR DETECTION OF TOXOPLASMOSIS IN SHEEP BY ELISA AND IFA TESTS. JOURNAL OF VETERINARY RESEARCH, 60(3), 219-223. SID. https://sid.ir/paper/35595/en

Vancouver: Copy

MORSHEDI A., MAHMOUDIAN A., AWAZ A.. DETERMINATION OF THE LOWEST VALNABLE TITER (CUT-OFF TITER) OF ANTITOXOPLASMA ANTIBODIES FOR DETECTION OF TOXOPLASMOSIS IN SHEEP BY ELISA AND IFA TESTS. JOURNAL OF VETERINARY RESEARCH[Internet]. 2005;60(3):219-223. Available from: https://sid.ir/paper/35595/en

IEEE: Copy

A. MORSHEDI, A. MAHMOUDIAN, and A. AWAZ, “DETERMINATION OF THE LOWEST VALNABLE TITER (CUT-OFF TITER) OF ANTITOXOPLASMA ANTIBODIES FOR DETECTION OF TOXOPLASMOSIS IN SHEEP BY ELISA AND IFA TESTS,” JOURNAL OF VETERINARY RESEARCH, vol. 60, no. 3, pp. 219–223, 2005, [Online]. Available: https://sid.ir/paper/35595/en

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