PROTEOME ANALYSIS OF WHEAT SEED EMBRYO (TRITIUM AESTIVUM) IN TOLERANT AND SUSCEPTIBLE CULTIVARS UNDER DROUGHT STRESS DURING EARLY GERMINATION PHASE

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SHAYANFAR ALI; TAVAKOL AFSHARI REZA; ALIZADEH HOUSHANG; RASOULNIA ABOLRAHMAN

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Journal: Iranian Journal of Field Crop Science Year:2015 | Volume:46 | Issue:2 Page(s): 0-0

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Title

PROTEOME ANALYSIS OF WHEAT SEED EMBRYO (TRITIUM AESTIVUM) IN TOLERANT AND SUSCEPTIBLE CULTIVARS UNDER DROUGHT STRESS DURING EARLY GERMINATION PHASE

Author(s)

SHAYANFAR ALI | TAVAKOL AFSHARI REZA | ALIZADEH HOUSHANG | RASOULNIA ABOLRAHMAN | Issue Writer Certificate

Keywords

EMBRYO SEEDQ2

GERMINATIONQ2

PROTEOMICSQ2

WHEATQ2

Abstract

Germination is one the most important growth phases in plants that is sensitive to drought stress extremely. GERMINATION divided in to three phases although before third phases, many activities (radicle protrusion) initiated. Many of metabolical activity occurred specially in the first phase since seed embryo is an alive organ, although differences in protein patterns happen in this phase. This study was carried out to evaluate the effect of drought stress on protein patterns in the first phase of GERMINATION. In order to analyze the effects of drought stress (-12 bar) on WHEAT embryo proteome (Sardari and Qods respectively tolerant and susceptible cultivar), factorial experiment was conducted in a randomized completely block design with three replications. Drought stress diminished GERMINATION percentage and caused adverse effects on the proteins pattern. Two-dimensional gel electrophoresis analysis of WHEAT embryo axis showed 1000 spots were reproducible that 25 spots between treatments indicated significant differences at 5% level. By comparing tolerant and susceptible cultivar, in control condition,, 22 and 6 spots, and in stress condition, 21 and 10 spots were down and up regulated, respectively. The results indicated that frequency of protein under drought stress depends on severity, amount of stress, and imbibition time.

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APA: Copy

SHAYANFAR, ALI, TAVAKOL AFSHARI, REZA, ALIZADEH, HOUSHANG, & RASOULNIA, ABOLRAHMAN. (2015). PROTEOME ANALYSIS OF WHEAT SEED EMBRYO (TRITIUM AESTIVUM) IN TOLERANT AND SUSCEPTIBLE CULTIVARS UNDER DROUGHT STRESS DURING EARLY GERMINATION PHASE. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 46(2), 0-0. SID. https://sid.ir/paper/357729/en

Vancouver: Copy

SHAYANFAR ALI, TAVAKOL AFSHARI REZA, ALIZADEH HOUSHANG, RASOULNIA ABOLRAHMAN. PROTEOME ANALYSIS OF WHEAT SEED EMBRYO (TRITIUM AESTIVUM) IN TOLERANT AND SUSCEPTIBLE CULTIVARS UNDER DROUGHT STRESS DURING EARLY GERMINATION PHASE. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2015;46(2):0-0. Available from: https://sid.ir/paper/357729/en

IEEE: Copy

ALI SHAYANFAR, REZA TAVAKOL AFSHARI, HOUSHANG ALIZADEH, and ABOLRAHMAN RASOULNIA, “PROTEOME ANALYSIS OF WHEAT SEED EMBRYO (TRITIUM AESTIVUM) IN TOLERANT AND SUSCEPTIBLE CULTIVARS UNDER DROUGHT STRESS DURING EARLY GERMINATION PHASE,” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 46, no. 2, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/357729/en

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