Molecular and bioinformatics analysis of regulatory upstream region of leptin and SIGLEC5 genes in association with production and reproduction traits in Holstein cattle

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Hoseinpour Kol Mahaleh M.; FARHADI A.; RAHIMI MIANJI GH.; GHOLIZADEH M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Animal Production Research Year:2019 | Volume:8 | Issue:3 Page(s): 73-85

Download Full-Text

Persian Verion

View:

257

Download:

Cites:

Information Journal Paper

Title

Molecular and bioinformatics analysis of regulatory upstream region of leptin and SIGLEC5 genes in association with production and reproduction traits in Holstein cattle

Author(s)

Hoseinpour Kol Mahaleh M. | FARHADI A. | RAHIMI MIANJI GH. | GHOLIZADEH M. | Issue Writer Certificate

Keywords

ProductionQ1

ReproductionQ2

Holstein cattleQ2

LeptinQ2

SIGLEC5Q1

Abstract

The aim of this study was the molecular and bioinformatics analysis of regulatory upstream region of Leptin and SIGLEC5 genes in association with Production and reProduction traits in Holstein cattle. For this purpose, 300 blood samples were collected randomly and DNA was extracted. Two fragments of 251 and 406 bp from regulatory upstream regions of Leptin and SIGLEC5 genes were amplified by PCR and genotyping was done by SSCP method. One sample from each banding pattern was sequenced and bioinformatics analysis were done by BioEdit and DNASIS MAX softwares. The SIGLEC5 locus was monomorphic, whereas, four banding patterns of A, B, C and D with frequencies of 0. 25, 0. 36, 0. 22 and 0. 17 were observed in Leptin locus, respectively. Statistical analysis showed that Leptin gene polymorphism was significantly (P<0. 05) associated with milk lipid and status of parturition, so that cows with banding pattern A had the highest milk fat and cows with banding pattern D showed lowest milk fat. Also, cows with banding pattern D had the best status of parturition (easy birth) (P<0. 05). The bioinformatics analysis showed 14 and nine motifs in SIGLEC5 and Leptin genes, respectively. Therefore, according to the results of this study, Leptin gene can be suggested to improve Production and reProduction traits. In addition, complementary studies on other regions of SIGLEC5 gene to find relations between Production and reProduction traits in Holstein cattle are highly recommended.

Cites

No record.

References

No record.

Cite

APA: Copy

Hoseinpour Kol Mahaleh, M., FARHADI, A., RAHIMI MIANJI, GH., & GHOLIZADEH, M.. (2019). Molecular and bioinformatics analysis of regulatory upstream region of leptin and SIGLEC5 genes in association with production and reproduction traits in Holstein cattle. ANIMAL PRODUCTION RESEARCH, 8(3 ), 73-85. SID. https://sid.ir/paper/365416/en

Vancouver: Copy

Hoseinpour Kol Mahaleh M., FARHADI A., RAHIMI MIANJI GH., GHOLIZADEH M.. Molecular and bioinformatics analysis of regulatory upstream region of leptin and SIGLEC5 genes in association with production and reproduction traits in Holstein cattle. ANIMAL PRODUCTION RESEARCH[Internet]. 2019;8(3 ):73-85. Available from: https://sid.ir/paper/365416/en

IEEE: Copy

M. Hoseinpour Kol Mahaleh, A. FARHADI, GH. RAHIMI MIANJI, and M. GHOLIZADEH, “Molecular and bioinformatics analysis of regulatory upstream region of leptin and SIGLEC5 genes in association with production and reproduction traits in Holstein cattle,” ANIMAL PRODUCTION RESEARCH, vol. 8, no. 3 , pp. 73–85, 2019, [Online]. Available: https://sid.ir/paper/365416/en

Related Journal Papers