Development of new primers based on gapdh gene for Cercospora species and new host and fungus records for Iran

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAKHSHI M.; ZARE R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MYCOLOGIA IRANICA Year:2020 | Volume:7 | Issue:1 Page(s): 63-82

Download Full-Text

Persian Verion

View:

317

Download:

198

Cites:

Information Journal Paper

Title

Development of new primers based on gapdh gene for Cercospora species and new host and fungus records for Iran

Author(s)

BAKHSHI M. | ZARE R. | Issue Writer Certificate

Keywords

BiodiversityQ1

cercosporoid fungiQ1

leaf spotQ1

MycosphaerellaceaeQ1

new primerQ1

Abstract

ITS region and protein coding genes such as actA, cmdA, gapdh, his3, rpb2, tef1 and tub2 have been applied to investigate the molecular phylogeny of Cercospora species in recent years. Although gapdh is an informative gene for species delimitation in the genus, difficult amplification of this locus with available primers limits its use for Cercospora. Therefore, in this study, novel primers including GpdF-Cer (5’-TTC ATY GAG CCM CAC TAC GCT-3’ ) and GpdR-Cer (5’-RTC GGT GAC KRC GAG VAC-3’ ) were developed to supplement previously published primers for the amplification of gapdh. Besides, in a taxonomic survey on the genus Cercospora in Iran based on consolidated species concept, leaf samples with leaf spot symptoms were collected and Cercospora isolates were characterized based on a combination of morphological features and sequence data from the ITS, actA, cmdA, gapdh, his3 and tef1 loci. Seventeen species of the genus Cercospora were recognized, of which C. mercurialis on Mercurialis annua is confirmed for the first time, for Iran (Asia) mycobiota using sequence data of six genomic loci. Several new hosts are recorded for C. apii (one), C. beticola (one), C. cf. flagellaris (10), C. gamsiana (one), C. iranica (one), Cercospora sp. G (three) and Cercospora sp. T (four). Thus, new host families were added to the host range of C. beticola (Brassicaceae), C. cf. flagellaris (Lamiaceae, Polygonaceae, Vitaceae), and Cercospora sp. T (Lamiaceae, Plantaginaceae, Rosaceae) in the world.

Cites

No record.

References

No record.

Cite

APA: Copy

BAKHSHI, M., & ZARE, R.. (2020). Development of new primers based on gapdh gene for Cercospora species and new host and fungus records for Iran. MYCOLOGIA IRANICA, 7(1 ), 63-82. SID. https://sid.ir/paper/366364/en

Vancouver: Copy

BAKHSHI M., ZARE R.. Development of new primers based on gapdh gene for Cercospora species and new host and fungus records for Iran. MYCOLOGIA IRANICA[Internet]. 2020;7(1 ):63-82. Available from: https://sid.ir/paper/366364/en

IEEE: Copy

M. BAKHSHI, and R. ZARE, “Development of new primers based on gapdh gene for Cercospora species and new host and fungus records for Iran,” MYCOLOGIA IRANICA, vol. 7, no. 1 , pp. 63–82, 2020, [Online]. Available: https://sid.ir/paper/366364/en

Related Journal Papers