Study of genetic variation among some Gossypium spp. genotypes available in Iran using ISSR molecular marker

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Tajik Khaveh Zahra; IRANBAKHSH ALIREZA; VAFAIE TABAR MOSAREZA; EBADI MOSTAFA

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Journal: Rostaniha Year:2020 | Volume:21 | Issue:2 (60) Page(s): 264-277

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Information Journal Paper

Title

Study of genetic variation among some Gossypium spp. genotypes available in Iran using ISSR molecular marker

Author(s)

Tajik Khaveh Zahra | IRANBAKHSH ALIREZA | VAFAIE TABAR MOSAREZA | EBADI MOSTAFA | Issue Writer Certificate

Keywords

Cotton variationsQ1

cultivarQ1

diploidQ2

genetic diversityQ1

MalvaceaeQ2

tetraploidQ1

Abstract

The current study was conducted to explore genetic variations among several cotton species, including Gossypium hirsutum cultivar Varamin, G. barbadense cultivar T14, G. herbaceum cultivar Arya, hybrid of G. hirsutum cultivar Varamin × G. barbadense cultivar T14 (V×T14 ), and a hybrid of G. hirsutum cultivar Varamin × G. herbaceum cultivar Arya (V×A) using inter-simple sequence repeats (ISSR). In this study, a total of 60 bands were amplified using four ISSR marker primers. As a result, 57 bands showed polymorphism. The results showed a high level of diversity in the studied varieties. The highest percentage of polymorphism was related to ISSR4 primer with ACACACACACACACACT sequence (100% ) and the lowest percentage was related to ISSR1 primer with AGAGAGAGAGAGAGAGC sequence (92. 8% ). Cluster analysis showed three major groups. The first group consisted of Arya and Varamin, the second group was comprised of V×A, T14 which showed a high genetic similarity indicating T14 originates from Varamin cultivar, and the third group contained V×T14. Overall, the results showed that, ISSR markers can be effectively used to study the genetic diversity of cotton.

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APA: Copy

Tajik Khaveh, Zahra, IRANBAKHSH, ALIREZA, VAFAIE TABAR, MOSAREZA, & EBADI, MOSTAFA. (2020). Study of genetic variation among some Gossypium spp. genotypes available in Iran using ISSR molecular marker. ROSTANIHA, 21(2 (60) ), 264-277. SID. https://sid.ir/paper/367640/en

Vancouver: Copy

Tajik Khaveh Zahra, IRANBAKHSH ALIREZA, VAFAIE TABAR MOSAREZA, EBADI MOSTAFA. Study of genetic variation among some Gossypium spp. genotypes available in Iran using ISSR molecular marker. ROSTANIHA[Internet]. 2020;21(2 (60) ):264-277. Available from: https://sid.ir/paper/367640/en

IEEE: Copy

Zahra Tajik Khaveh, ALIREZA IRANBAKHSH, MOSAREZA VAFAIE TABAR, and MOSTAFA EBADI, “Study of genetic variation among some Gossypium spp. genotypes available in Iran using ISSR molecular marker,” ROSTANIHA, vol. 21, no. 2 (60) , pp. 264–277, 2020, [Online]. Available: https://sid.ir/paper/367640/en

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