Investigation of Optimum Conditions of Sporogenesis and Cultivation of Clostridium novyi: cause of Gas gangrene

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Abedi Jafari F.; Pilehchian Langerod R.; ABDOLI A.; SOLEIMANI N.; HOSSEINI S.M.

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Journal: Veterinary Research & Biological Products Year:2021 | Volume:33 | Issue:4 (129) Page(s): 10-20

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Title

Investigation of Optimum Conditions of Sporogenesis and Cultivation of Clostridium novyi: cause of Gas gangrene

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Keywords

Clostridium novyi type BQ1

SporeQ1

Bacterial countQ1

Bacterial cultureQ1

Candidate for solid tumor treatmentQ1

Abstract

Clostridium novyi is endoSpore-forming, obligate anaerobic bacteria of the class clostridia. Cultivation of this bacterium in common culture is one of the problems with this bacterium. It is pathogenic, causing a wide variety of diseases in man and animals the Spore of this bacterium has also received much attention in the treatment of cancer due to its ability to grow in anaerobic conditions inside solid tumors. In this study, the optimum growth conditions for vegetative and Spore form was investigated. Bacterial culture was investigated in different solid media including BHI, Blood Agar, F medium and NP medium in vegetative form. Also, a suitable Spore media was introduced, the Spore purification method, an easy method for Bacterial count and Spore aspiration were investigated. The best rich media for C. novyi in vegetative form are F and NP media. That is, by adding horse blood to these media culture, the growth be lux but is not a sufficient factor for growth. There was an almost linear relationship between bacterial absorption and Bacterial count for Clostridium novyi. Also, the difference in the number of Spores germination which heating at 80 ° C for 15 minutes was significant.

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APA: Copy

Abedi Jafari, F., Pilehchian Langerod, R., ABDOLI, A., SOLEIMANI, N., & HOSSEINI, S.M.. (2021). Investigation of Optimum Conditions of Sporogenesis and Cultivation of Clostridium novyi: cause of Gas gangrene. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 33(4 (129) ), 10-20. SID. https://sid.ir/paper/371362/en

Vancouver: Copy

Abedi Jafari F., Pilehchian Langerod R., ABDOLI A., SOLEIMANI N., HOSSEINI S.M.. Investigation of Optimum Conditions of Sporogenesis and Cultivation of Clostridium novyi: cause of Gas gangrene. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2021;33(4 (129) ):10-20. Available from: https://sid.ir/paper/371362/en

IEEE: Copy

F. Abedi Jafari, R. Pilehchian Langerod, A. ABDOLI, N. SOLEIMANI, and S.M. HOSSEINI, “Investigation of Optimum Conditions of Sporogenesis and Cultivation of Clostridium novyi: cause of Gas gangrene,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 33, no. 4 (129) , pp. 10–20, 2021, [Online]. Available: https://sid.ir/paper/371362/en

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