THE POSSIBILITY OF IN VITRO HAPLOID PRODUCTION IN CHICKPEA (CICER ARIETINUM L.)

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VESAL S.R.; BAGHERI A.; SAFARNEZHAD A.

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Journal: Journal of Water and Soil Science Year:2002 | Volume:6 | Issue:2 Page(s): 76-88

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Title

THE POSSIBILITY OF IN VITRO HAPLOID PRODUCTION IN CHICKPEA (CICER ARIETINUM L.)

Author(s)

VESAL S.R. | BAGHERI A. | SAFARNEZHAD A. | Issue Writer Certificate

Keywords

CALLUS INDUCTIONQ4

REGENERATIONQ4

ANTHER CULTUREQ4

CHICKPEAQ3

HAPLOIDQ4

Abstract

In order to investigate the androgenic response of CHICKPEA cultivars, two Iranian CHICKPEA cultivars, Pirooz and Karaj 12-60-31, were used in this study. After 7 to 10 days of cold pretreatment of flower buds, another containing uninucleate stage of microspores were placed aseptically on MS medium supplemented with various combinations of growth regulators of 2,4-D (1,2 and 3 mg/l) and kinetin (0.1, 0.2 and 0.5 mg/I). Callus REGENERATION achieved using MS and modified media with various hormones and different sucrose concentrations.The results indicated that the callus initiation was significantly affected by 2,4-D and kinetin concentrations, and that increasing these hormones reduced CALLUS INDUCTION. The best response obtained on media with the lowest concentration levels of 2,4-D and kinetin (1 and 0.2 mg/1, respectively). A highly significant genotypic effect and a genotype x 2,4-D interaction were detected, which proved that Pirooz response was the best. Callus differentiation and organogenesis occurred in MS medium supplemented with NAA, BA and 3% sucrose. Mature embryos also obtained in modified Blayd plus 0.5 mg/l kinetin and 10% sucrose. Cytological studies revealed the presence of HAPLOID cells with chromosome variation in the anther derived callus. Therefore, optimizing the hormone levels of different basal media with a particular sucrose concentration may improve HAPLOID REGENERATION in CHICKPEA. It seems a further study should be carried out to characterize calli from induction to REGENERATION and to determine the effect of cold pretreatment, the results of which could be used to improve ANTHER CULTURE response of CHICKPEA.

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APA: Copy

VESAL, S.R., BAGHERI, A., & SAFARNEZHAD, A.. (2002). THE POSSIBILITY OF IN VITRO HAPLOID PRODUCTION IN CHICKPEA (CICER ARIETINUM L.). WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), 6(2), 76-88. SID. https://sid.ir/paper/373174/en

Vancouver: Copy

VESAL S.R., BAGHERI A., SAFARNEZHAD A.. THE POSSIBILITY OF IN VITRO HAPLOID PRODUCTION IN CHICKPEA (CICER ARIETINUM L.). WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES)[Internet]. 2002;6(2):76-88. Available from: https://sid.ir/paper/373174/en

IEEE: Copy

S.R. VESAL, A. BAGHERI, and A. SAFARNEZHAD, “THE POSSIBILITY OF IN VITRO HAPLOID PRODUCTION IN CHICKPEA (CICER ARIETINUM L.),” WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), vol. 6, no. 2, pp. 76–88, 2002, [Online]. Available: https://sid.ir/paper/373174/en

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