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Information Journal Paper

Title

Seroprevalence and Molecular Study of Toxoplasma Infection in Domestic Chickens from Khorramabad, Iran

Pages

  130-135

Abstract

 BACKGROUND: Toxoplasma gondii is an intracellular protozoan parasite that can infect most species of warm-blooded animals, including birds and humans. Because of feeding habits of Domestic chickens, prevalence of Toxoplasma infection in free-range chickens is considered as a suitable indicator of environmental distribution of oocysts. OBJECTIVES: The present study was designed to investigate the Seroprevalence of Toxoplasma infection in Domestic chickens from Khorramabad and compare the results obtained from serological and molecular methods. METHODS: In total, 97 serum samples were randomly obtained from Domestic chickens and examined for the presence of anti-Toxoplasma antibodies using modified agglutination test (MAT). Fifty grams of muscles (mixture of breast and heart) and whole brain from seropositive chickens were separately homogenized and examined by PCR which targets the repeated element (RE) of the parasite. RESULTS: Anti-Toxoplasma antibodies were observed in 21 of 97 (21. 64%) sera. T. gondii DNA was detected in 10 out of 21 (47. 61%) seropositive chickens (with titres of ≥ 1: 20). The low agreement between serological and molecular results can be explained by several factors such as possibility of cross-reactions in MAT and/or limited sample size in PCR. CONCLUSIONS: These results indicate that Domestic chickens may have an important role as a source of infection for cats and individuals living in rural areas.

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    APA: Copy

    Ahmadi, Seyed Foad, Zarifi, Ozra, Shokrani, Hamidreza, & Norouzian, Hassan. (2020). Seroprevalence and Molecular Study of Toxoplasma Infection in Domestic Chickens from Khorramabad, Iran. JOURNAL OF VETERINARY RESEARCH, 75(2 ), 130-135. SID. https://sid.ir/paper/375055/en

    Vancouver: Copy

    Ahmadi Seyed Foad, Zarifi Ozra, Shokrani Hamidreza, Norouzian Hassan. Seroprevalence and Molecular Study of Toxoplasma Infection in Domestic Chickens from Khorramabad, Iran. JOURNAL OF VETERINARY RESEARCH[Internet]. 2020;75(2 ):130-135. Available from: https://sid.ir/paper/375055/en

    IEEE: Copy

    Seyed Foad Ahmadi, Ozra Zarifi, Hamidreza Shokrani, and Hassan Norouzian, “Seroprevalence and Molecular Study of Toxoplasma Infection in Domestic Chickens from Khorramabad, Iran,” JOURNAL OF VETERINARY RESEARCH, vol. 75, no. 2 , pp. 130–135, 2020, [Online]. Available: https://sid.ir/paper/375055/en

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