The Role of NO in the Opium-Induced Bowel Dysfunction in the Mice

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Dabili Nasrabadi Amin; ARAB HOSSEIN ALI; Fatemi Ardestani Syyed Ahmad; Hassanpor Hossein

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Journal: Journal of Veterinary Research Year:2020 | Volume:75 | Issue:2 Page(s): 252-261

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Title

The Role of NO in the Opium-Induced Bowel Dysfunction in the Mice

Author(s)

Dabili Nasrabadi Amin | ARAB HOSSEIN ALI | Fatemi Ardestani Syyed Ahmad | Hassanpor Hossein | Issue Writer Certificate

Keywords

OpiumQ1

Nitric oxideQ2

MiceQ2

IntestineQ2

Isolated organQ1

Abstract

BACKGROUND: Opioids and Nitric oxide (NO) are functionally linked in the regulation of intestinal motility. OBJECTIVES: To investigate the role of NO in the Opium induced bowel dysfunction in Mice. METHODS: Sixty-six male Mice received incrementally doses of the following treatments in six groups for 5 consecutive days: 1) Opium (0. 2, 0. 3, 0. 4, 0. 5 and 0. 6mg/30g/day), 2) N-nitro-L-arginine methyl ester (L-NAME, 5, 7. 5, 10, 15 and 20mg/kg/day), 3) L-arginine (5-20mg/kg/day), 4) Opium+L-NAME, 5) Opium+L-arginine and 6) distilled water. At the end of the treatment, the abdomen was opened; some pieces of duodenal and proximal colon were taken to determine NO synthase (NOS) expression and nitrite levels, and some isolated rings from those parts of small and large Intestine were prepared and transferred to the organ bath system to study intestinal motility. RT-PCR was used to determine the NOS gene expression. To determine the small intestinal transit, 30 Mice in six groups, were used for oral administration of charcoal+gum in vivo. RESULTS: Opium decreased amplitude of the duodenum and ileum contractions, but increased frequency of duodenal and mid colon contractions (P<0. 05). While the gene expression of inducible, neuronal and endothelial NOS was increased in colon (P<0. 05), a reduced neuronal and endothelial NOS gene expression was shown in duodenum. The charcoal+gum transit was decreased in Opium-treated animals compared to the control group (19. 9%). However, Larginine increased this transit while L-NAME decreased it. CONCLUSIONS: Opium induced intestinal smooth muscle spasms, which result in the decreased intestinal movements. The alterations in NOS gene expression may be a compensation mechanism against Opium-induced intestinal dysfunction.

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APA: Copy

Dabili Nasrabadi, Amin, ARAB, HOSSEIN ALI, Fatemi Ardestani, Syyed Ahmad, & Hassanpor, Hossein. (2020). The Role of NO in the Opium-Induced Bowel Dysfunction in the Mice. JOURNAL OF VETERINARY RESEARCH, 75(2 ), 252-261. SID. https://sid.ir/paper/376276/en

Vancouver: Copy

Dabili Nasrabadi Amin, ARAB HOSSEIN ALI, Fatemi Ardestani Syyed Ahmad, Hassanpor Hossein. The Role of NO in the Opium-Induced Bowel Dysfunction in the Mice. JOURNAL OF VETERINARY RESEARCH[Internet]. 2020;75(2 ):252-261. Available from: https://sid.ir/paper/376276/en

IEEE: Copy

Amin Dabili Nasrabadi, HOSSEIN ALI ARAB, Syyed Ahmad Fatemi Ardestani, and Hossein Hassanpor, “The Role of NO in the Opium-Induced Bowel Dysfunction in the Mice,” JOURNAL OF VETERINARY RESEARCH, vol. 75, no. 2 , pp. 252–261, 2020, [Online]. Available: https://sid.ir/paper/376276/en

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