The effects of nano-ZnO and nano-TiO2 on expression of Phenylalanine ammonia lyase (PAL) and Cinnamyl alcohol dehydrogenase (CAD) genes in cell culture of Linum usitatissimum L.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KARIMZADEH F.; HADDAD R.; GAROOSI GH.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2019 | Volume:14 | Issue:2 Page(s): 171-177

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

314

Download:

Cites:

Information Journal Paper

Title

The effects of nano-ZnO and nano-TiO2 on expression of Phenylalanine ammonia lyase (PAL) and Cinnamyl alcohol dehydrogenase (CAD) genes in cell culture of Linum usitatissimum L.

Author(s)

KARIMZADEH F. | HADDAD R. | GAROOSI GH. | Issue Writer Certificate

Keywords

Cinnamyl alcohol dehydrogenaseQ2

Cell cultureQ1

FlaxQ1

NanoparticlesQ1

Phenylalanine ammonia lyaseQ1

gene expressionQ2

Abstract

The Flaxseed (Linum usitatissimum L) is recognized as an important crop and the rich source of useful fatty acids, protein, dietary fiber and lignans. Lignans as antioxidant compounds are an important group of secondary metabolites that have important biological activities, such as anti-cancer effects in humans. In this study, the expression of Phenylalanine ammonia lyase (PAL) and Cinnamyl alcohol dehydrogenase (CAD) genes in the lignan biosynthesis pathway were investigated under the influence of nano-ZnO and nano-TiO2 in Flax Cell culture. For this purpose, a factorial experiment in a completely randomized design with three replications were done to study the effects of of nano-ZnO and nano-TiO2. Nano-ZnO at 0, 30, 60 and 120 mg/L and nano-TiO2 at 0, 50, 100 and 150 mg/L were added to Cell cultures and samples were collected at 0, 24, 48, and 72 h after adding Nanoparticles. The gene expression analysis were studied using Real-Time PCR. The highest expression of PAL gene was observed at the concentrations of 30 and 60 mg/L, and the lowest expression was observed at the concentration of 120 mg/L of nano-ZnO. Nano-TiO2 had no significant effect on PAL gene expression, but the use of 150 mg/L nano-TiO2 led to increased expression of CAD at 48 and 72h. CAD expression increased at 60 mg/L concentration of nano ZnO at all intervals and the lowest expression of CAD was observed at 120 mg/L nano ZnO. The results of this study showed that zinc Nanoparticles are effective in increasing the expression of both PAL and CAD genes, while the nano-TiO2 only affect the expression of CAD and has no effect on the expression of PAL expression.

Cites

No record.

References

No record.

Cite

APA: Copy

KARIMZADEH, F., HADDAD, R., & GAROOSI, GH.. (2019). The effects of nano-ZnO and nano-TiO2 on expression of Phenylalanine ammonia lyase (PAL) and Cinnamyl alcohol dehydrogenase (CAD) genes in cell culture of Linum usitatissimum L.. MODERN GENETICS JOURNAL (MGJ), 14(2 ), 171-177. SID. https://sid.ir/paper/377315/en

Vancouver: Copy

KARIMZADEH F., HADDAD R., GAROOSI GH.. The effects of nano-ZnO and nano-TiO2 on expression of Phenylalanine ammonia lyase (PAL) and Cinnamyl alcohol dehydrogenase (CAD) genes in cell culture of Linum usitatissimum L.. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2019;14(2 ):171-177. Available from: https://sid.ir/paper/377315/en

IEEE: Copy

F. KARIMZADEH, R. HADDAD, and GH. GAROOSI, “The effects of nano-ZnO and nano-TiO2 on expression of Phenylalanine ammonia lyase (PAL) and Cinnamyl alcohol dehydrogenase (CAD) genes in cell culture of Linum usitatissimum L.,” MODERN GENETICS JOURNAL (MGJ), vol. 14, no. 2 , pp. 171–177, 2019, [Online]. Available: https://sid.ir/paper/377315/en

Related Journal Papers