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Information Journal Paper

Title

Effect of type and concentration of growth regulators on the proliferation and marcotting of Withania coagulans medical plant

Pages

  287-294

Abstract

 In order to provide a rapid and useful method for propagation of Withania coagulans medical plant under in-vitro condition was conducted based on completely randomized design experience in tissue culture laboratory of Agricultural Research Center of Khorasan Razavi Province. At first, sterilized seeds are placed in MS media (collected from natural habitat in Sistan and Baluchistan Province). Then for shoot regeneration internode subculture placed in MS media, including Benzylaminopurine (BAP) in 5 levels (0, 0. 5, 1, 1. 5, 2 mg/L). In step 2, for root regeneration placed in MS media in including indole-3-butyric acid (IBA) in 4 levels 0. 5, 1, 1. 5, 2 mg/L and Naphthaleneacetic acid (NAA) in 0. 5, 1, 1. 5, 2 mg/L), also, control treatment (non-application of hormone) was considered. Finally, in order to adapt to seedling, they were kept in an adaptive room for 30 days. The highest proliferation index, number of branches, and number of leaf belong to 0. 5 mg/L BAP treatment and the best concentration of growth regulators with 90% marcotting was in the treatment of IBA at 0. 5 mg/L concentration. Finally, by transfer of regenerated explants to the soil, 80 percent of them survived. In general, for the production of strong and succulent seedlings, the use of BAP and IBA at 0. 5 mg/L concentration is recommended for branching and marcotting, respectively.

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    APA: Copy

    Ghahremani, Aysan, GANJI MOGHADAM, EBRAHIM, TATARI, MARYAM, & KHOSROYAR, SUSAN. (2020). Effect of type and concentration of growth regulators on the proliferation and marcotting of Withania coagulans medical plant. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 51(2 ), 287-294. SID. https://sid.ir/paper/378715/en

    Vancouver: Copy

    Ghahremani Aysan, GANJI MOGHADAM EBRAHIM, TATARI MARYAM, KHOSROYAR SUSAN. Effect of type and concentration of growth regulators on the proliferation and marcotting of Withania coagulans medical plant. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2020;51(2 ):287-294. Available from: https://sid.ir/paper/378715/en

    IEEE: Copy

    Aysan Ghahremani, EBRAHIM GANJI MOGHADAM, MARYAM TATARI, and SUSAN KHOSROYAR, “Effect of type and concentration of growth regulators on the proliferation and marcotting of Withania coagulans medical plant,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 51, no. 2 , pp. 287–294, 2020, [Online]. Available: https://sid.ir/paper/378715/en

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