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Information Journal Paper

Title

Effect of a Phenolic compounds-rich feedstuff on expression of amylase and β Actin genes in liver and pancreas tissues of broilers

Pages

  79-88

Keywords

AgeQ1

Abstract

 This study was conducted to determine the Amylase gene expression in liver and pancreas tissues of broiler chicks fed with two levels of Oak acorn (OA) fruit. A total of 264 broiler chicks were fed a control diet (without oak) and diets containing 15 and 20% OA. At the end of starter and finisher period, 6 birds from each treatment were randomly selected and Tissue samples were taken from their pancreas and liver. REST and SAS software were used to analyze gene expression. The 2004 Bestkeeper software was also used to determine the sustainability of β-actin gene expression. Results showed that β-actin gene was not affected by sex, Age and experimental treatments. Thus, it was used to normalize the gene expression data. At 21d of Age, the level of mRNA of the pancreatic Amylase gene was significantly higher in broilers fed with 15% OA compared to the control group (p<0. 05). On d 42, significant differences in expression of liver and pancreas Amylase gene were not observed (p>0. 05). Also, feeding birds with diet containing 20% OA increased the relative weight of pancreas (p<0. 05). In conclusion, the results showed that Amylase gene expression in birds fed diets containing 15% OA significantly increased at 21d of Age. In other cases, genes expression was not influenced by experimental treatments.

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    • Cite

    APA: Copy

    Moradalipour, Asma, Muhaghegh Dolatabady, mostafa, HOUSHMAND, MOHAMMAD, & Zarrin, Mousa. (2019). Effect of a Phenolic compounds-rich feedstuff on expression of amylase and β Actin genes in liver and pancreas tissues of broilers. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 50(1 ), 79-88. SID. https://sid.ir/paper/378721/en

    Vancouver: Copy

    Moradalipour Asma, Muhaghegh Dolatabady mostafa, HOUSHMAND MOHAMMAD, Zarrin Mousa. Effect of a Phenolic compounds-rich feedstuff on expression of amylase and β Actin genes in liver and pancreas tissues of broilers. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2019;50(1 ):79-88. Available from: https://sid.ir/paper/378721/en

    IEEE: Copy

    Asma Moradalipour, mostafa Muhaghegh Dolatabady, MOHAMMAD HOUSHMAND, and Mousa Zarrin, “Effect of a Phenolic compounds-rich feedstuff on expression of amylase and β Actin genes in liver and pancreas tissues of broilers,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 50, no. 1 , pp. 79–88, 2019, [Online]. Available: https://sid.ir/paper/378721/en

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