Evaluation of a Multiplex Polymerase Chain Reaction for the Simultaneous Detection of Vibrio spp. in Vegetables and Water

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AHARI HAMED; Shoja Gharehbagh Sonia; ANVAR AMIRALI; Aftoum Mahtab; KHANI MOHAMMADREZA

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Journal: Iranian Journal of Veterinary Medicine Year:2020 | Volume:14 | Issue:4 Page(s): 386-393

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Title

Evaluation of a Multiplex Polymerase Chain Reaction for the Simultaneous Detection of Vibrio spp. in Vegetables and Water

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Keywords

Foodborne outbreaksQ1

Multiplex PCRQ1

Simultaneous detectionQ1

VegetablesQ1

Vibrio sppQ1

Abstract

BACKGROUND: Several Foodborne outbreaks associated with the consumption of Vegetables have been reported which involved Vibrio spp. as causative agents. Conventional methods of detecting these microorganisms are time-consuming. Therefore, the development of techniques for rapid detection seems to be of paramount importance. OBJECTIVES: The present study recommends a rapid and reliable method for the detection of Vibrio cholera (V. chol-era), V. parahaemolyticus, V. vulnificus, and V. alginolyticus. Moreover, the results are compared with the conventional plate culture method. METHODS: The conventional bacteriological tests were conducted to detect Vibrio spp. in Vegetables and their surrounding water. The samples were also subjected to a newly developed multiplex polymerase chain reaction (PCR) using five specific genes, including VC-Rmm of V. cholerae, VP-MmR of V. parahaemolyticus, VV-Rmm of V. vulnificus, V. al2-MmR of V. alginolyticus, and VM-F for all the four isolates. RESULTS: The presence of V. alginolyticus and V. vulnificus was confirmed by amplifying the specific regions of 412 bp for V. vulnificus and 144 bp for V. alginolyticus. The results demonstrated that V. cholerae and V. parahaemolyticus were not detected in Multiplex PCR, which was consistent with the findings of conventional plating methods. CONCLUSIONS: Obtained results revealed that the designed Multiplex PCR assay is a reliable, rapid, and cost-effective method for the Simultaneous detection of Vibrio spp.

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APA: Copy

AHARI, HAMED, Shoja Gharehbagh, Sonia, ANVAR, AMIRALI, Aftoum, Mahtab, & KHANI, MOHAMMADREZA. (2020). Evaluation of a Multiplex Polymerase Chain Reaction for the Simultaneous Detection of Vibrio spp. in Vegetables and Water. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 14(4 ), 386-393. SID. https://sid.ir/paper/378780/en

Vancouver: Copy

AHARI HAMED, Shoja Gharehbagh Sonia, ANVAR AMIRALI, Aftoum Mahtab, KHANI MOHAMMADREZA. Evaluation of a Multiplex Polymerase Chain Reaction for the Simultaneous Detection of Vibrio spp. in Vegetables and Water. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2020;14(4 ):386-393. Available from: https://sid.ir/paper/378780/en

IEEE: Copy

HAMED AHARI, Sonia Shoja Gharehbagh, AMIRALI ANVAR, Mahtab Aftoum, and MOHAMMADREZA KHANI, “Evaluation of a Multiplex Polymerase Chain Reaction for the Simultaneous Detection of Vibrio spp. in Vegetables and Water,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 14, no. 4 , pp. 386–393, 2020, [Online]. Available: https://sid.ir/paper/378780/en

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