Identification of self-incompatibility alleles in some of Yazd province almond genotypes by using PCR method

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Abouei Mitra; KAMALI KAZEM; Soluki Mahmood; FAHMIDEH LEILA; IMANI ALI

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Journal: Iranian Journal of Horticultural Science Year:2021 | Volume:51 | Issue:4 Page(s): 797-808

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Title

Identification of self-incompatibility alleles in some of Yazd province almond genotypes by using PCR method

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Keywords

AlmondQ1

molecular markerQ2

PCRQ1

S-alleleQ1

self-compatibilityQ1

Abstract

One of the problems in Almond production is self-incompatibility in this plant, which is considered as an important point of breeding. Self-incompatibility causes non-uniformity of harvesting time as well as some of garden management and pollination problems. Most cultivars of Almonds have gametophytic self-incompatibility that is controlled by a multi-allelic gene locus. The fertilization inhibitor factor in this phenomenon, pollen tube growth stops in the middle of the style. The purpose of this research was identification and determination of the selfcompatible genotype in the Yazd province. in this investigation better genotypes of Almond were collected and after DNA extraction was done, in order to detect S alleles in different Almond and some hybrid genotypes, the specific primer pairs, including AS1II-AmyC5R, ConF-ConR and Cebador2-Cebador8, were used in the polymerase chain reaction. In polymerase chain reaction, using the AS1II-AmyC5R and Cebador2-Cebador8 primers, the Sf allele with the size of 1200 base pairs was detected. Using the ConF-ConR pair of primer, the S1, S2, S3, S10, S11, S23, and S31 alleles were detected in the self-incompatible samples. Using AS1II-AmyC5R pair of primer, the known alleles of S3, Sf, S2, S1, S5, S10 S11 S23, and S13 were detected. The other bands obtained from the PCR were related to the known self-in-compatibility alleles that might be considered as new alleles. According to the obtained results, S1, S2, S3, and S11 alleles had the highest frequency and S5, S23, S10, S13 and S31 alleles respectively had the lowest frequency.

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APA: Copy

Abouei, Mitra, KAMALI, KAZEM, Soluki, Mahmood, FAHMIDEH, LEILA, & IMANI, ALI. (2021). Identification of self-incompatibility alleles in some of Yazd province almond genotypes by using PCR method. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 51(4 ), 797-808. SID. https://sid.ir/paper/380624/en

Vancouver: Copy

Abouei Mitra, KAMALI KAZEM, Soluki Mahmood, FAHMIDEH LEILA, IMANI ALI. Identification of self-incompatibility alleles in some of Yazd province almond genotypes by using PCR method. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2021;51(4 ):797-808. Available from: https://sid.ir/paper/380624/en

IEEE: Copy

Mitra Abouei, KAZEM KAMALI, Mahmood Soluki, LEILA FAHMIDEH, and ALI IMANI, “Identification of self-incompatibility alleles in some of Yazd province almond genotypes by using PCR method,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 51, no. 4 , pp. 797–808, 2021, [Online]. Available: https://sid.ir/paper/380624/en

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