Optimization in vitro micropropagation of peony (Paeonia mascula)

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Mahdavi Fikijvar Edris; ZAKIZADEH HEDAYAT; MOZAFFARIAN VALIOLLAH; REZADOOST HASSAN

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Journal: Iranian Journal of Horticultural Science Year:2020 | Volume:51 | Issue:1 Page(s): 153-164

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Title

Optimization in vitro micropropagation of peony (Paeonia mascula)

Author(s)

Mahdavi Fikijvar Edris | ZAKIZADEH HEDAYAT | MOZAFFARIAN VALIOLLAH | REZADOOST HASSAN | Issue Writer Certificate

Keywords

In vitro cultureQ2

organogenesisQ1

somatic embryogenesisQ2

Abstract

This research was conducted in two stages to optimize in vitro propagation of Persian wild paeony. Stage 1: effect of BAP (0, 0. 5, 1 and 2 mg. l-1), NAA (0, 0. 1, 0. 2 and 0. 3 mg. l-1) and GA3 (0 and 0. 5 mg. l-1) on apical bud of Paeonia mascula and stage 2: effect of BAP (0, 0. 5, 1 and 2 mg. l-1) and NAA (0, 0. 25, 0. 5 and 1 mg. l-1) were studied on embryonic callus induction on young leaves of Paonia mascula. At stage 1: the highest shoots number (5. 38) was obtained on media containing 1 mg. l-1 BAP + 0. 1 mg. l-1 NAA+ 0. 5 mg. l-1 GA3. The results showed that increasing BAP level caused decreasing of shoot length. At stage 2: maximum of compact embryonic callus (17. 93%) and somatic embryo number per explant (7. 11) were obtained on 2 mg. l-1 BAP and 0. 5mg. l-1 NAA. For callus proliferation, calli were maintained on MS medium containing 2 mg. l-1 BAP, 0. 1 mg. l-1 NAA and 0. 5 mg. l-1 GA3. Maximum rooting percentage (70. 51%) and root number (0. 0. 02) were obtained with 0. 25 mg. l-1 NAA.

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APA: Copy

Mahdavi Fikijvar, Edris, ZAKIZADEH, HEDAYAT, MOZAFFARIAN, VALIOLLAH, & REZADOOST, HASSAN. (2020). Optimization in vitro micropropagation of peony (Paeonia mascula). IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 51(1 ), 153-164. SID. https://sid.ir/paper/380692/en

Vancouver: Copy

Mahdavi Fikijvar Edris, ZAKIZADEH HEDAYAT, MOZAFFARIAN VALIOLLAH, REZADOOST HASSAN. Optimization in vitro micropropagation of peony (Paeonia mascula). IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2020;51(1 ):153-164. Available from: https://sid.ir/paper/380692/en

IEEE: Copy

Edris Mahdavi Fikijvar, HEDAYAT ZAKIZADEH, VALIOLLAH MOZAFFARIAN, and HASSAN REZADOOST, “Optimization in vitro micropropagation of peony (Paeonia mascula),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 51, no. 1 , pp. 153–164, 2020, [Online]. Available: https://sid.ir/paper/380692/en

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