Effect of P19 gene expression on transcription rate and production of recombinant human tissue plasminogen (rtPA) in tobacco (nicotiana benthamiana)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHARAFI YOUSEF; JALALI JAVARAN MOKHTAR; SABET MOHAMMAD SADEGH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2020 | Volume:12 | Issue:2 Page(s): 103-128

Download Full-Text

Persian Verion

View:

775

Download:

Cites:

Information Journal Paper

Title

Effect of P19 gene expression on transcription rate and production of recombinant human tissue plasminogen (rtPA) in tobacco (nicotiana benthamiana)

Author(s)

SHARAFI YOUSEF | JALALI JAVARAN MOKHTAR | SABET MOHAMMAD SADEGH | Issue Writer Certificate

Keywords

AgrobacteriumQ2

Transient ExpressionQ1

Molecular FarmingQ1

Silencing SuppressorQ1

Abstract

Objective Tissue plasminogen activator is one of the most important drugs in the treatment of heart disease. This drug is produced in the expression system as a recombinant protein that has high production costs. Transient Expression system is very suitable for protein expression because of its high expression, high speed, low cost and no spatial effect. Posttranscriptional silencing has been shown to affect expression levels. Therefore, the aim of this study was to investigate the effect of simultaneous expression of P19 Silencing Suppressor gene on Transient Expression of recombinant tissue plasminogen activator (rtPA) at transcriptional and protein levels in Nicotiana benthamiana. Material and methods To serve this purpose, the expression proportion of injected Agrobacterium tumefaciens containing a binary vector pCAMBIA1304-rtPA with Agrobacterium containing pCAMBIA1304-P19 have been studied comparison with the expression level from Agrobacterium containing only the binary vector pCAMBIA1304-rtPA. Leaf samples were prepared on 4, 7, and 10 day post-inoculation with Agrobacterium. Transcription and then protein levels were calculated using the Real Time PCR and ELISA tests. Results The results of Real Time PCR test showed that rtPA transcript increased in the presence of P19. Also, 4 days after plant inoculation, the highest transcript levels were obtained from p19 and rtPA genes. ELISA results showed that the expression of rtPA protein in the presence of P19 was 89 and 84 μ g. g-1 leaf weight at the 7 and 10 day after inoculation, respectively. This expression was 12 and 15% higher than of when Agrobacterium-containing pCAMBIA1304-rtPA vector alone was used, respectively. Conclusion The results showed that the use of Transient Expression method could be a suitable method for rtPA protein production.

Cites

No record.

References

No record.

Cite

APA: Copy

SHARAFI, YOUSEF, JALALI JAVARAN, MOKHTAR, & SABET, MOHAMMAD SADEGH. (2020). Effect of P19 gene expression on transcription rate and production of recombinant human tissue plasminogen (rtPA) in tobacco (nicotiana benthamiana). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 12(2 ), 103-128. SID. https://sid.ir/paper/381124/en

Vancouver: Copy

SHARAFI YOUSEF, JALALI JAVARAN MOKHTAR, SABET MOHAMMAD SADEGH. Effect of P19 gene expression on transcription rate and production of recombinant human tissue plasminogen (rtPA) in tobacco (nicotiana benthamiana). JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2020;12(2 ):103-128. Available from: https://sid.ir/paper/381124/en

IEEE: Copy

YOUSEF SHARAFI, MOKHTAR JALALI JAVARAN, and MOHAMMAD SADEGH SABET, “Effect of P19 gene expression on transcription rate and production of recombinant human tissue plasminogen (rtPA) in tobacco (nicotiana benthamiana),” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 12, no. 2 , pp. 103–128, 2020, [Online]. Available: https://sid.ir/paper/381124/en

Related Journal Papers