Effect of Different Elicitor Treatments on Hairy Root of Medicinal Plant Poppies (Papaver somniferum L.)

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Siahmansour sh.; ISMAILI A.; NAZARIAN FIROUZABADI F.

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Journal: Plant Productions Year:2018 | Volume:41 | Issue:1 Page(s): 29-42

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Information Journal Paper

Title

Effect of Different Elicitor Treatments on Hairy Root of Medicinal Plant Poppies (Papaver somniferum L.)

Author(s)

Siahmansour sh. | ISMAILI A. | NAZARIAN FIROUZABADI F. | Issue Writer Certificate

Keywords

AbioticQ1

Agrobacterium rhizogenesisQ1

BiomassQ1

BioticQ1

TransgenicQ1

Abstract

Background and Objectives As a source of alkaloids, Poppy (Papaver somniferum L. ), is one of the most important plants in the field of pharmaceutical industry. Various methods including use of elicitors, adding precursor, optimizing culture, culture of hairy roots and its metabolites engineering are used to increase the production of secondary metabolites in plant cell cultures. In recent years, cultivation of hairy roots, to the production of valuable metabolites in a number of species of medicinal plants, has taken on commercial scale; but in most cases, this technique produces low amount of alkaloid in commercial scale. Hence, it is needed to optimize the production rate using biological and nonbiological elicitors. Materials and Methods This experiment was conducted in 2015 in biotechnology laboratory of Lorestan University, Khorramabad, Iran. Sterile explants were prepared from seedling and were then inoculated with Agrobacterium. Inoculated explants were kept in a dark condition for 2 days and then washed and transferred to glasses containing ½ MS medium complemented with cefotaxime. Finally, the glasses were kept in tissue culture room until production of hairy roots. The produced hairy roots were used as explants for elicitors' experiments in flask containers. In this study, in order to increase Biomass of hairy roots, 4 types of elicitor treatments (salicylic acid, silver nitrate, copper sulphate and yeast extract) were evaluated in separate experiments based on a completely randomized design with 3 replicates. Results Confirmation of Transgenic hairy roots was tested by PCR using the rolC gene primers to ensure the removal of bacteria from the produced roots. Results of PCR products were separated by electrophoresis and expected fragments were observed in gel. The results showed that the concentration of 5. 0 mg/mL of yeast extract elicitor had the most impact on morphological traits. Totally, results showed that Biotic elicitors had better effects in comparison to Biotic/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ABiotic elicitors. Also, high concentrations of elicitors in all Biotic and Biotic/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ABiotic cases had a negative impact on Biomass of hairy roots. Discussion It was shown that Biotic elicitors had better effects than Biotic/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ABiotic elicitors. The remarkable result of this study was that the high concentration of elicitors had negative effects on Biomass hairy root production in poppies plants. It seems that bio-active compounds elicitors induce the plant responses but high concentration of Biotic and Biotic/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ABiotic elicitors may cause cell death and browning of hairy root tissues.

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APA: Copy

Siahmansour, sh., ISMAILI, A., & NAZARIAN FIROUZABADI, F.. (2018). Effect of Different Elicitor Treatments on Hairy Root of Medicinal Plant Poppies (Papaver somniferum L.). THE PLANT PRODUCTION (SCIENTIFIC JOURNAL OF AGRICULTURE), 41(1 ), 29-42. SID. https://sid.ir/paper/382857/en

Vancouver: Copy

Siahmansour sh., ISMAILI A., NAZARIAN FIROUZABADI F.. Effect of Different Elicitor Treatments on Hairy Root of Medicinal Plant Poppies (Papaver somniferum L.). THE PLANT PRODUCTION (SCIENTIFIC JOURNAL OF AGRICULTURE)[Internet]. 2018;41(1 ):29-42. Available from: https://sid.ir/paper/382857/en

IEEE: Copy

sh. Siahmansour, A. ISMAILI, and F. NAZARIAN FIROUZABADI, “Effect of Different Elicitor Treatments on Hairy Root of Medicinal Plant Poppies (Papaver somniferum L.),” THE PLANT PRODUCTION (SCIENTIFIC JOURNAL OF AGRICULTURE), vol. 41, no. 1 , pp. 29–42, 2018, [Online]. Available: https://sid.ir/paper/382857/en

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