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Information Journal Paper

Title

Effect of cell-free supernatant of Lactobacillus reuteri on the growth rate of toxigenic Fusarium oxysporum in vitro

Pages

  11-20

Abstract

 Lactobacillus reuteri is a probiotic bacteria that produces, a wide-range of antimicrobial substance, “ reuterin” in the presence of glycerol. The present study was aimed to evaluate the efficacy of Lactobacillus reuteri against the growth of Fusarium oxysporum. So, the cell-free culture supernatant (CFCS) of Lactobacillus reuteri was prepared by centrifugation (8000 rpm for 15 min) of bacterial overnight culture, with and without 2% glycerol medium. Inhibition effects, evaluated by two methods: micro-well dilution (both of CFCSs: two-fold dilution series (100, … 6. 25 μ l); 10 μ l fungal spore suspension (2×106 spores/ml); PDB medium) and Tube culture (both of CFCSs: (80, 60 μ l); 10 μ l fungal spore suspension (2×106 spores/ml); SDB medium). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of CFCS obtained from glycerol-medium, was determined as 60μ l. The CFCS obtained from glycerol-free medium had no inhibitory effect on F. oxysprom growth. The results of the present study revealed that the CFCS of Lactobacillus reuteri obtained from medium with 2% glycerol, probably due to the presence of reuterin, is able to inhibit the growth of toxigenic Fusarium oxysporum and can be used in the feed and food industry as a potential bio-controller against toxigenic Fusarium species.

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    APA: Copy

    Rahimi Kakolaki, Maryam, & OMIDI, ARASH. (2020). Effect of cell-free supernatant of Lactobacillus reuteri on the growth rate of toxigenic Fusarium oxysporum in vitro. NEW FINDINGS IN VETERINARY MICROBIOLOGY, 3(1 ), 11-20. SID. https://sid.ir/paper/389047/en

    Vancouver: Copy

    Rahimi Kakolaki Maryam, OMIDI ARASH. Effect of cell-free supernatant of Lactobacillus reuteri on the growth rate of toxigenic Fusarium oxysporum in vitro. NEW FINDINGS IN VETERINARY MICROBIOLOGY[Internet]. 2020;3(1 ):11-20. Available from: https://sid.ir/paper/389047/en

    IEEE: Copy

    Maryam Rahimi Kakolaki, and ARASH OMIDI, “Effect of cell-free supernatant of Lactobacillus reuteri on the growth rate of toxigenic Fusarium oxysporum in vitro,” NEW FINDINGS IN VETERINARY MICROBIOLOGY, vol. 3, no. 1 , pp. 11–20, 2020, [Online]. Available: https://sid.ir/paper/389047/en

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