DISTRIBUTION AND GENETIC DIVERSITY OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES IN KERMAN PROVINCE

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AHMADI S.; AFSHARIFAR A.R.; NIAZI A.; IZADPANAH K.A.

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Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:4 (172) Page(s): 351-371

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Title

DISTRIBUTION AND GENETIC DIVERSITY OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES IN KERMAN PROVINCE

Author(s)

AHMADI S. | AFSHARIFAR A.R. | NIAZI A. | IZADPANAH K.A. | Issue Writer Certificate

Keywords

CITRUS TRISTEZA VIRUSQ3

CLOSTEROVIRUSQ3

KERMANQ3

CITRUSQ2

Abstract

Despite the widespread occurrence of CITRUS TRISTEZA VIRUS (CTV) in some regions of southern and northern Iran, there is no information about the distribution and genetic variation of CTV isolates in KERMAN province. In this study the prevalence and the molecular variability of CTV isolates from four CITRUS growing regions of this province, including Bam, Jiroft, Orzuieh and Hossein Abad, were investigated. Total RNA from CTV infected bark and midrib of different CITRUS trees was extracted and used for amplification of CP and K17 region of the polymerase genes of the CTV genome, by RT-PCR. The results of ELISA showed that out of 203 random CITRUS samples collected from the four regions in KERMAN province; about 50% were infected with CTV. The rate of CTV infection in samples of Jiroft, Bam, Hossein Abad and Orzuieh, was 60%, 56%, 50% and 45%, respectively. PCR results showed the presence of expected 672 and 409 bp fragments for CP in 9 samples and K17 region of polymerase genes in 9 samples of the CTV genome respectively in 9 samples of KERMAN province isolates. The resulted PCR products were inserted into pTZ57R/T plasmid, cloned in E.coli strain DH5a, sequenced, and compared with the related sequences from the GenBank by BLAST search program. The results revealed that on the basis of CP and a part of the polymerase genes, CTV isolates of KERMAN province have a higher homology with the California stem pitting (SY568) and Japanese seedling yellows (NUagA) isolates. However, six isolates of KERMAN province (KBA, KG-9, KG-10, KG-11, KH-10, KS-7), showed distinct differences with other CTV isolates in four amino acids in CP gene and formed a separate branch in dendrogram.

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APA: Copy

AHMADI, S., AFSHARIFAR, A.R., NIAZI, A., & IZADPANAH, K.A.. (2007). DISTRIBUTION AND GENETIC DIVERSITY OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES IN KERMAN PROVINCE. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(4 (172)), 351-371. SID. https://sid.ir/paper/391496/en

Vancouver: Copy

AHMADI S., AFSHARIFAR A.R., NIAZI A., IZADPANAH K.A.. DISTRIBUTION AND GENETIC DIVERSITY OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES IN KERMAN PROVINCE. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(4 (172)):351-371. Available from: https://sid.ir/paper/391496/en

IEEE: Copy

S. AHMADI, A.R. AFSHARIFAR, A. NIAZI, and K.A. IZADPANAH, “DISTRIBUTION AND GENETIC DIVERSITY OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES IN KERMAN PROVINCE,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 4 (172), pp. 351–371, 2007, [Online]. Available: https://sid.ir/paper/391496/en

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