Micropropagation of Chestnut (Castanea sativa Mill. ) Affected by Plant Growth Regulators under In Vitro Conditions

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TAFAZOLI MEHRCEDEH; HOSSEINI NASR SEYED MOHAMMAD; JALILVAND HAMID; Tafazoli Mahya

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Journal Paper

Paper Information

Journal: ECOLOGY OF IRANIAN FOREST Year:2021 | Volume:9 | Issue:17 Page(s): 114-122

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Information Journal Paper

Title

Micropropagation of Chestnut (Castanea sativa Mill. ) Affected by Plant Growth Regulators under In Vitro Conditions

Author(s)

TAFAZOLI MEHRCEDEH | HOSSEINI NASR SEYED MOHAMMAD | JALILVAND HAMID | Tafazoli Mahya | Issue Writer Certificate

Keywords

Callus-shootingQ2

ChestnutQ1

MicropropagationQ2

Plant growth regulatorQ1

RootingQ1

Abstract

Chestnut as an indigenous and valuable forest species exists in some forests of Guilan province, which the area of its stands is decreasing due to the lack of natural regeneration and socioeconomic problems as well as ink and blight diseases; therefore, Chestnut’ s asexual propagation through tissue culture methods can be effective in protecting this species and preventing its extinction. The aim of this study was to use the Micropropagation method in order to produce complete Chestnut plantlets by identifying the best explants and Plant growth regulators. For this purpose, seeds were collected from Visroud site, sterilized, soaked in boiling water and cultured on B5 medium, and then explants were taken from germinated seeds. Leaf, stem and root explants from in vitro seedlings of Chestnut were cultured on B5 containing cytokinins (TDZ and BA) with concentrations of 0. 02, 0. 2, 1 and 2 mg/l and auxins (IBA, NAA, and IAA) with concentrations of 0. 1, 0. 2, 1 and 2 mg/l. Results showed that the best response was obtained from the leaf explants and TDZ (0. 2 mg/l) and IBA (2 mg/l) had the highest percentage of Callus-shooting (32. 4%) and Rooting (80%), respectively. The survival of seedlings outside the laboratory was 80%. Considering the importance of Chestnut species in economic and environmental terms, the present study can be useful for the implementation of reclamation management to protect, develop and prevent extinction of this species.

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APA: Copy

TAFAZOLI, MEHRCEDEH, HOSSEINI NASR, SEYED MOHAMMAD, JALILVAND, HAMID, & Tafazoli, Mahya. (2021). Micropropagation of Chestnut (Castanea sativa Mill. ) Affected by Plant Growth Regulators under In Vitro Conditions. ECOLOGY OF IRANIAN FOREST, 9(17 ), 114-122. SID. https://sid.ir/paper/391601/en

Vancouver: Copy

TAFAZOLI MEHRCEDEH, HOSSEINI NASR SEYED MOHAMMAD, JALILVAND HAMID, Tafazoli Mahya. Micropropagation of Chestnut (Castanea sativa Mill. ) Affected by Plant Growth Regulators under In Vitro Conditions. ECOLOGY OF IRANIAN FOREST[Internet]. 2021;9(17 ):114-122. Available from: https://sid.ir/paper/391601/en

IEEE: Copy

MEHRCEDEH TAFAZOLI, SEYED MOHAMMAD HOSSEINI NASR, HAMID JALILVAND, and Mahya Tafazoli, “Micropropagation of Chestnut (Castanea sativa Mill. ) Affected by Plant Growth Regulators under In Vitro Conditions,” ECOLOGY OF IRANIAN FOREST, vol. 9, no. 17 , pp. 114–122, 2021, [Online]. Available: https://sid.ir/paper/391601/en

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