First report on the association of a phytoplasma with cowpea phyllody disease in Bavi county, Khuzestan province

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GHODOUM PARIZIPOUR M.H.; Shahriari A.Gh.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2019 | Volume:54 | Issue:4 Page(s): 349-352

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Title

First report on the association of a phytoplasma with cowpea phyllody disease in Bavi county, Khuzestan province

Author(s)

GHODOUM PARIZIPOUR M.H. | Shahriari A.Gh. | Issue Writer Certificate

Keywords

cowpeaQ2

Khuzestan provinceQ3

phytoplasmaQ1

Abstract

Phytolasmas are important phloem-restricted plant pathogens which are classified into prokaryotes kingdom and mollicutes class and annually cause considerable loss to crops, ornamentals and trees (Bertaccini et al. 2014). During 2017-18, cowpea fields of Bavi county in Khuzestan province were inspected and cowpea phyllody disease was observed. The clear symptoms of the disease included phyllody, leaf size reduction and stem fasciation (Fig. 1-A). Total DNA was extracted from 34 symptomatic plant samples and 12 healthy plants. In order to detect phytoplasma in DNA samples, direct PCR using P1/P7 primer pair (Deng & Hiruki 1991) and nested PCR using R16F2n/R16FR2 primer pair (Gundersen & Lee 1996) were used and expected fragments of 1830 and 1240 bp were amplified, respectively (Fig. 1-B). The large fragment belonging to the sample collected from Bavi county was isolated, purified and sequenced. Nucleotide analysis of the obtained sequence in NCBI using BLAST software showed the highest (99%) identity to the phytoplasma associated with cowpea phyllody in West Azarbaijan province (KC633094), Vinca virescence (AY500817), Columbia Basin potato purple top (AY692279) and Candidatus phytoplasma trifolii (KX773529). Additionally, 99% identity to 14 phytoplasmas reported from Iran such as Cucurbita pepo (KP119494), cucumber phyllody (JF508516) and tomato big bud (JF508512) was observed. The nearly complete sequence of 16SrRNA of cowpea phyllody phytoplasma was deposited in GenBank under the accession number of MK088178. This is the first report on the association of a phytoplasma with cowpea phyllody disease in Khuzestan province. Recently, a phytoplasma has been detected among cowpea plants with symptoms including phyllody, leaf size reduction and stem fasciation in West Azarbaijan province which showed highest similarity to clover proliferation group (16SrVI) (Gharouni Kardani & Jamshidi 2018). It seems that the presence of phytoplasma in this province is a real threat to cultivated crops. Symptoms of phyllody, leaf size reduction and stem fasciation were also found in cowpea fields of Ahvaz and Dezful counties and identification of the phytoplasma associated with these symptoms is under investigation.

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APA: Copy

GHODOUM PARIZIPOUR, M.H., & Shahriari, A.Gh.. (2019). First report on the association of a phytoplasma with cowpea phyllody disease in Bavi county, Khuzestan province. IRANIAN JOURNAL OF PLANT PATHOLOGY, 54(4 ), 349-352. SID. https://sid.ir/paper/399699/en

Vancouver: Copy

GHODOUM PARIZIPOUR M.H., Shahriari A.Gh.. First report on the association of a phytoplasma with cowpea phyllody disease in Bavi county, Khuzestan province. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2019;54(4 ):349-352. Available from: https://sid.ir/paper/399699/en

IEEE: Copy

M.H. GHODOUM PARIZIPOUR, and A.Gh. Shahriari, “First report on the association of a phytoplasma with cowpea phyllody disease in Bavi county, Khuzestan province,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 54, no. 4 , pp. 349–352, 2019, [Online]. Available: https://sid.ir/paper/399699/en

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