Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks

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Jahangirzadeh Khiavi Shahin; ZAMANI ZABIHOLLAH; Fatahi Mohamadreza; Ashourpour Masoumeh

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Paper Information

Journal: JOURNAL OF PLANT GENETIC RESEARCH Year:2018 | Volume:5 | Issue:1 Page(s): 77-86

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Title

Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks

Author(s)

Jahangirzadeh Khiavi Shahin | ZAMANI ZABIHOLLAH | Fatahi Mohamadreza | Ashourpour Masoumeh | Issue Writer Certificate

Keywords

Chloroplast universal primerQ1

Restriction enzymeQ1

Malus SppQ1

HaplotypeQ1

PCR-RFLPQ2

Abstract

The chloroplastic DNA of 64 accessions of apple (Malus Spp. ) (54 Iranian genotypes, five commercial cultivars and five rootstocks) were analyzed to reveal their Haplotypes by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Approximately 4320 bp of the chloroplast genome was analyzed, by using two Chloroplast universal primer pairs and two Restriction enzymes (EcoRI and MseI). All the mutations detected were insertion-deletions been in the range between 10-40 bp. The combination of all the mutations resulted to six Haplotypes (H1, H2, H3, H4, H5 and H6) in the studied apple accessions. It was defined that the Iranian apple rootstock 'Gami-Almasi' had a specific Haplotype form all the other studied accessions. It was approved that cpDNA diversity data can be considered for phylogenetic studies in this plant and the polymorphism determined in cytoplasmic genome by PCR-RFLP method can help to understand the maternal inheritance in apple.

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APA: Copy

Jahangirzadeh Khiavi, Shahin, ZAMANI, ZABIHOLLAH, Fatahi, Mohamadreza, & Ashourpour, Masoumeh. (2018). Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks. JOURNAL OF PLANT GENETIC RESEARCH, 5(1 ), 77-86. SID. https://sid.ir/paper/400247/en

Vancouver: Copy

Jahangirzadeh Khiavi Shahin, ZAMANI ZABIHOLLAH, Fatahi Mohamadreza, Ashourpour Masoumeh. Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks. JOURNAL OF PLANT GENETIC RESEARCH[Internet]. 2018;5(1 ):77-86. Available from: https://sid.ir/paper/400247/en

IEEE: Copy

Shahin Jahangirzadeh Khiavi, ZABIHOLLAH ZAMANI, Mohamadreza Fatahi, and Masoumeh Ashourpour, “Comparison of Chloroplast DNA Diversity in Some Iranian Apple Genotypes, Commercial Cultivars and Rootstocks,” JOURNAL OF PLANT GENETIC RESEARCH, vol. 5, no. 1 , pp. 77–86, 2018, [Online]. Available: https://sid.ir/paper/400247/en

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