مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

351
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Inulin content and expression of the genes involved in inulin biosynthesis in root and cell suspension culture of Chicory (Cichorium intybus L. )

Pages

  115-127

Abstract

 Plant cell suspension cultures are widely used as an in vitro culture technique for primary and secondary metabolites production. Fructans are one of the herbal metabolites that considerably accumulate in the Asteraceae family, especially in chicory root. In this study, the amount of glucose, fructose, sucrose, 1kestose, nystose, Inulin, mean degree of polymerization (mDP) of Inulin and expression of genes involved in Inulin biosynthesis (1-SST, 1-FFT, 1-FEHI, and 1-FEHII) were investigated in the roots and the cell suspension cultures of chicory. The results showed that Inulin quantity and the mDP of Inulin in cell suspension cultures are less than roots. Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) analysis showed that the gene expression of 1-FFT was significantly down-regulated in the cell suspension cultures compared to the root. For other genes, no significant differences were detected between the roots and cell suspension cultures. High quantity of fructose and glucose in cell suspension culture indicates the high activity of fructan hydrolyzed enzymes (FEHI and 1-FEHII), therefore, lower Inulin content and mean degree of polymerization could be due to low expression of 1-FFT gene and high activity of 1-FEHs. The results showed that the quantity of Inulin in cell suspension culture is lower than roots, but cell suspension cultures have desirable capabilities in Inulin production in a short time.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    MOHAMMADI, FATEMEH, Naghavi, Mohamad Reza, Peighambari, Seyed Ali, & Khosravi Dehaghi, Nafiseh. (2020). Inulin content and expression of the genes involved in inulin biosynthesis in root and cell suspension culture of Chicory (Cichorium intybus L. ). IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 51(3 ), 115-127. SID. https://sid.ir/paper/407647/en

    Vancouver: Copy

    MOHAMMADI FATEMEH, Naghavi Mohamad Reza, Peighambari Seyed Ali, Khosravi Dehaghi Nafiseh. Inulin content and expression of the genes involved in inulin biosynthesis in root and cell suspension culture of Chicory (Cichorium intybus L. ). IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2020;51(3 ):115-127. Available from: https://sid.ir/paper/407647/en

    IEEE: Copy

    FATEMEH MOHAMMADI, Mohamad Reza Naghavi, Seyed Ali Peighambari, and Nafiseh Khosravi Dehaghi, “Inulin content and expression of the genes involved in inulin biosynthesis in root and cell suspension culture of Chicory (Cichorium intybus L. ),” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 51, no. 3 , pp. 115–127, 2020, [Online]. Available: https://sid.ir/paper/407647/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top