RAPD-PCR and Drug Resistance Pattern of Staphylococcus aureus Isolates Recovered from Companion and Wild Birds

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Bagheri Sina; PEIGHAMBARI SEYED MOSTAFA; SOLTANI MOHAMMAD; MALEKAN MOHAMMAD

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Journal: Iranian Journal of Veterinary Medicine Year:2019 | Volume:13 | Issue:4 Page(s): 355-364

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Title

RAPD-PCR and Drug Resistance Pattern of Staphylococcus aureus Isolates Recovered from Companion and Wild Birds

Author(s)

Bagheri Sina | PEIGHAMBARI SEYED MOSTAFA | SOLTANI MOHAMMAD | MALEKAN MOHAMMAD | Issue Writer Certificate

Keywords

Companion birdsQ1

drug resistanceQ1

molecular epidemiologyQ1

RAPD-PCRQ1

Staphylococcus aureusQ1

Abstract

BACKGROUND: Staphylococcus aureus is a highly versatile pathogen of a large number of domestic animals, including avian species. There is limited information about S. aureus isolated from companion and wild birds in Iran. OBJECTIVES: The aim of this study was to determine drug resistance and random-amplified polymorphic DNA-PCR (RAPD-PCR) pattern of S. aureus isolated from birds referred to the pet birds’ clinic of University of Tehran. METHODS: During the study period, 53 isolates of S. aureus were recovered from Companion birds of various species using standard bacteriologic procedures and the respective drug resistance patterns were determined for a panel of 30 antimicrobial agents by agar disk-diffusion method. RAPD-PCR was performed with two different 10-bp oligonucleotide primers in a duplex-PCR procedure. RESULTS: The findings of this study demonstrated that S. aureus resistance to oxacillin, clindamycin and methicillin were 58, 53 and 53%, respectively. The multi-drug resistance (MDR) was found among all isolates. The MDR pattern was variable and ranged from 0 to 17 drugs. In total, all 53 isolates generated 43 different resistance patterns. In RAPD-PCR, five different patterns of A, B, C, D and E were found. Among 53 isolates, 20, 62, 3, 9 and 3% belonged to RAPD patterns of A, B, C, D and E, respectively. CONCLUSIONS: This study showed the widespread antimicrobial resistance among S. aureus isolated from pet birds; in particular, the presence of MRSA isolates. The value of RAPD-PCR for epidemiologic monitoring of S. aureus in pet birds also was noticed.

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APA: Copy

Bagheri, Sina, PEIGHAMBARI, SEYED MOSTAFA, SOLTANI, MOHAMMAD, & MALEKAN, MOHAMMAD. (2019). RAPD-PCR and Drug Resistance Pattern of Staphylococcus aureus Isolates Recovered from Companion and Wild Birds. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 13(4 ), 355-364. SID. https://sid.ir/paper/409262/en

Vancouver: Copy

Bagheri Sina, PEIGHAMBARI SEYED MOSTAFA, SOLTANI MOHAMMAD, MALEKAN MOHAMMAD. RAPD-PCR and Drug Resistance Pattern of Staphylococcus aureus Isolates Recovered from Companion and Wild Birds. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2019;13(4 ):355-364. Available from: https://sid.ir/paper/409262/en

IEEE: Copy

Sina Bagheri, SEYED MOSTAFA PEIGHAMBARI, MOHAMMAD SOLTANI, and MOHAMMAD MALEKAN, “RAPD-PCR and Drug Resistance Pattern of Staphylococcus aureus Isolates Recovered from Companion and Wild Birds,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 13, no. 4 , pp. 355–364, 2019, [Online]. Available: https://sid.ir/paper/409262/en

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