INTRODUCING MOLECULAR MARKERS FOR LERNAEA CYPRINACEA AND LERNAEA CTENOPHARYNGODONI USING RAPD TECHNIQUE

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POURKAZEMI MOHAMMAD; ASADIAN HAMID; PAZOUKI J.; MASOUMIAN MAHMOUD; EBRAHIMZADEH MOUSAVI H.A.

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Journal: Iranian Scientific Fisheries Journal Year:2007 | Volume:16 | Issue:3 Page(s): 19-28

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Title

INTRODUCING MOLECULAR MARKERS FOR LERNAEA CYPRINACEA AND LERNAEA CTENOPHARYNGODONI USING RAPD TECHNIQUE

Author(s)

Keywords

RAPDQ3

PCRQ3

LERNAEA CYPRINACEAQ3

LERNAEA CTENOPHARYNGODONIQ3

GUILAN PROVINCEQ3

KHOUZESTAN PROVINCEQ3

Abstract

Molecular comparison of two parasites LERNAEA CYPRINACEA and LERNAEA CTENOPHARYNGODONI was carried out using RAPD (Random Amplified Polymorphic DNA) technique. A total of 43 Lernaea specimens belonging to the two species were collected from the Guilan and KHOUZESTAN PROVINCEs. DNA was extracted using the Phenol-chloroform method. The quality and quantity of DNA was assessed using 1% Agarose gel electrophoresis and spectrophotometer. Polymerase Chain Reaction (PCR) was conducted on the target DNA under specific conditions and PCR products were subjected to electrophoresis on polyacrylamide gels (6%). Polyacrylamide gels were stained using silver nitrate and DNA bands were analyzed with BioCapt software. The genetic analysis was conducted using POP GEN 32 software.Forty two primers, 10 nucleotides each were used for PCR reaction. Totally, 397 RAPD loci were counted on polyacrylamide gel where 349 identical loci were polymorphic of which some bands may be used as genetic markers for the identification of both Lernaea species. Data analysis on PCR products showed higher genetic variation (1.15%) of LERNAEA CTENOPHARYNGODONI in the GUILAN PROVINCE as compared to that of the Khouzestan (0.0%).However, genetic variation (27.46%) of LERNAEA CYPRINACEA in the KHOUZESTAN PROVINCE was 7.26 times higher than that of the GUILAN PROVINCE (3.78%). The two species showed a genetic differentiation of approximately 88%. Based on the observed molecular differences, we state that LERNAEA CTENOPHARYNGODONI is a genetically independent species from LERNAEA CYPRINACEA.

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References

IDENTIFICATION OF CRUSTACEAN PARASITES IN SOME FISHES OF MAHABAD RESERVOIR

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APA: Copy

POURKAZEMI, MOHAMMAD, ASADIAN, HAMID, PAZOUKI, J., MASOUMIAN, MAHMOUD, & EBRAHIMZADEH MOUSAVI, H.A.. (2007). INTRODUCING MOLECULAR MARKERS FOR LERNAEA CYPRINACEA AND LERNAEA CTENOPHARYNGODONI USING RAPD TECHNIQUE. IRANIAN SCIENTIFIC FISHERIES JOURNAL, 16(3), 19-28. SID. https://sid.ir/paper/4778/en

Vancouver: Copy

POURKAZEMI MOHAMMAD, ASADIAN HAMID, PAZOUKI J., MASOUMIAN MAHMOUD, EBRAHIMZADEH MOUSAVI H.A.. INTRODUCING MOLECULAR MARKERS FOR LERNAEA CYPRINACEA AND LERNAEA CTENOPHARYNGODONI USING RAPD TECHNIQUE. IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2007;16(3):19-28. Available from: https://sid.ir/paper/4778/en

IEEE: Copy

MOHAMMAD POURKAZEMI, HAMID ASADIAN, J. PAZOUKI, MAHMOUD MASOUMIAN, and H.A. EBRAHIMZADEH MOUSAVI, “INTRODUCING MOLECULAR MARKERS FOR LERNAEA CYPRINACEA AND LERNAEA CTENOPHARYNGODONI USING RAPD TECHNIQUE,” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 16, no. 3, pp. 19–28, 2007, [Online]. Available: https://sid.ir/paper/4778/en

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