USE OF RAPID SEROLOGICAL AND MOLECULAR METHODS IN DIFFERENTIATING BARLEY YELLOW DWARF VIRUSES

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RASTGOU M.; KHATABI B.; IAZDPANAH K.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2005 | Volume:41 | Issue:1 Page(s): 125-140

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Information Journal Paper

Title

USE OF RAPID SEROLOGICAL AND MOLECULAR METHODS IN DIFFERENTIATING BARLEY YELLOW DWARF VIRUSES

Author(s)

RASTGOU M. | KHATABI B. | IAZDPANAH K. | Issue Writer Certificate

Keywords

BYDV-PAVQ3

CYDV-RPVQ4

RAPID SEROLOGICAL AND MOLECULAR METHODSQ4

RTPCRQ2

IC-RT-PCRQ4

COCKTAIL-ELISAQ4

Abstract

Detection of barley yellow dwarf viruses (BYDVs) and Cereal yellow dwarf virus (CYDV) is very important for disease management. In the present study several serological and molecular methods for rapid detection of these viruses were compared. Among the serological methods that were used in this study, TPIA and COCKTAIL-ELISA had more advantages. TPIA needs few equipments and can be used widely in the field. COCKTAIL-ELISA was time-saving. Both methods can differentiate and detect serotypes. By RT-PCR with degenerate primers 400 bp and 1200 bp long segments for RPV and PAV, respectively, were amplified. Segments of 700 bp and 800 bp were amplified by using RPV and PAV specific primers, respectively. In this study, regular RT-PCR was unable to detect serotypes in crude saps of the infected plants, while IC-RT-PCR by using polyclonal antibodies of these two serotypes (to trap virus particles) was able to amplify the expected segments. Our findings suggest that TPIA and IC-RT-PCR can be used routinely with high efficiency for BYDVs and CYDV detection.

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APA: Copy

RASTGOU, M., KHATABI, B., & IAZDPANAH, K.. (2005). USE OF RAPID SEROLOGICAL AND MOLECULAR METHODS IN DIFFERENTIATING BARLEY YELLOW DWARF VIRUSES. IRANIAN JOURNAL OF PLANT PATHOLOGY, 41(1), 125-140. SID. https://sid.ir/paper/48155/en

Vancouver: Copy

RASTGOU M., KHATABI B., IAZDPANAH K.. USE OF RAPID SEROLOGICAL AND MOLECULAR METHODS IN DIFFERENTIATING BARLEY YELLOW DWARF VIRUSES. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2005;41(1):125-140. Available from: https://sid.ir/paper/48155/en

IEEE: Copy

M. RASTGOU, B. KHATABI, and K. IAZDPANAH, “USE OF RAPID SEROLOGICAL AND MOLECULAR METHODS IN DIFFERENTIATING BARLEY YELLOW DWARF VIRUSES,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 41, no. 1, pp. 125–140, 2005, [Online]. Available: https://sid.ir/paper/48155/en

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