INDUCTION OF RESISTANCE TO BARLEY YELLOW DWARF VIRUS (PAV) IN BREAD WHEAT USING POST-TRANSCRIPTIONAL GENE SILENCING (PTGS)

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YASSAIE M.; AFSHARIFAR A.R.; NIAZI A.; SALEHZADEH S.; IZADPANAH K.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2011 | Volume:47 | Issue:1 (185) Page(s): 67-82

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Title

INDUCTION OF RESISTANCE TO BARLEY YELLOW DWARF VIRUS (PAV) IN BREAD WHEAT USING POST-TRANSCRIPTIONAL GENE SILENCING (PTGS)

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Keywords

BARLEY YELLOW DWARF VIRUS PAVQ2

POST TRANSCRIPTIONAL GENE SILENCINGQ2

DNA CONSTRUCTQ2

RESISTANCEQ2

GENE SILENCING CONSTRUCTSQ2

Abstract

Barley and cereal yellow dwarf are the most important cereal diseases causing serious damages worldwide. Limited number of RESISTANCE and tolerance genes, their inefficiency and their complex inheritance justifies the adoption of the pathogen derived RESISTANCE approaches. In the present study, three types of constructs including sense, antisense and intron hairpin-loop (ihp-loop) were prepared from a sequence of BYDV-PAV polymerase gene and cloned into pWBVec8 binary vector. Agrobacterium-mediated transformation of mature embryos of bread wheat Alvand was carried out using the three types of constructs in the optimized culture media supplemented with hygromycin as a selective marker. The transformed explants were evaluated by ELISA, polymerase chain reaction, and dot blot assay using a nonradioactive probe. Artificial inoculation of plants was carried out employing BYDV-PAV viruliferous aphids. The average rates of transformation were evaluated as 1.8, 1.3 and zero percent for ihp-loop, sense and antisense constructs, respectively, based on ELISA values. Transformed plants remained healthy while control plants developed yellow dwarf symptoms after artificial inoculation of BYDV-PAV.

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APA: Copy

YASSAIE, M., AFSHARIFAR, A.R., NIAZI, A., SALEHZADEH, S., & IZADPANAH, K.. (2011). INDUCTION OF RESISTANCE TO BARLEY YELLOW DWARF VIRUS (PAV) IN BREAD WHEAT USING POST-TRANSCRIPTIONAL GENE SILENCING (PTGS). IRANIAN JOURNAL OF PLANT PATHOLOGY, 47(1 (185)), 67-82. SID. https://sid.ir/paper/48234/en

Vancouver: Copy

YASSAIE M., AFSHARIFAR A.R., NIAZI A., SALEHZADEH S., IZADPANAH K.. INDUCTION OF RESISTANCE TO BARLEY YELLOW DWARF VIRUS (PAV) IN BREAD WHEAT USING POST-TRANSCRIPTIONAL GENE SILENCING (PTGS). IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2011;47(1 (185)):67-82. Available from: https://sid.ir/paper/48234/en

IEEE: Copy

M. YASSAIE, A.R. AFSHARIFAR, A. NIAZI, S. SALEHZADEH, and K. IZADPANAH, “INDUCTION OF RESISTANCE TO BARLEY YELLOW DWARF VIRUS (PAV) IN BREAD WHEAT USING POST-TRANSCRIPTIONAL GENE SILENCING (PTGS),” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 47, no. 1 (185), pp. 67–82, 2011, [Online]. Available: https://sid.ir/paper/48234/en

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