SPECIES-SPECIFIC PCR IDENTIFICATION AND DETECTION OF PHYTOPHTHORA DRECHSLERI, P. CRYPTOGEA AND P. ERYTHROSEPTICA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOSTOWFIZADEH GHALAMFARSA R.; BANIHASHEMI Z.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2016 | Volume:51 | Issue:4 Page(s): 541-553

Download Full-Text

Persian Verion

View:

897

Download:

Cites:

Information Journal Paper

Title

SPECIES-SPECIFIC PCR IDENTIFICATION AND DETECTION OF PHYTOPHTHORA DRECHSLERI, P. CRYPTOGEA AND P. ERYTHROSEPTICA

Author(s)

MOSTOWFIZADEH GHALAMFARSA R. | BANIHASHEMI Z. | Issue Writer Certificate

Keywords

PHYTOPHTHORA DRECHSLERIQ2

PHYTOPHTHORA CRYPTOGEAQ1

PHYTOPHTHORA ERYTHROSEPTICAQ2

OOMYCOTAQ2

INTERNAL TRANSCRIBED SPACERQ2

CYTOCHROME C OXIDASE SUBUNIT IQ2

IDENTIFICATIONQ2

DETECTIONQ2

Abstract

PHYTOPHTHORA DRECHSLERI, P. cryptogea and P. erythroseptica are phylogenetically closely related Oomyceteous plant pathogens which are morphologically similar. In order to discriminate these taxa from each other and from species with convergent morphological characteristics a simple as well as a nested-PCR based method was developed. A collection of isolates of each species from different hosts representing world-wide diversity of species were examined for unique regions of nuclear as well as mitochondrial genes. Six candidate PCR primers were designed and calibrated for species-specific amplification of P. drechsleri based on the DNA sequences of rDNA INTERNAL TRANSCRIBED SPACER regions and the CYTOCHROME C OXIDASE SUBUNIT I, and also three candidate PCR primers specific for P. cryptogea and P. erythroseptica were designed and calibrated based on CYTOCHROME C OXIDASE SUBUNIT I. Studies showed that the best primer set for IDENTIFICATION of P. drechsleri was the combination of ITS-DF2 and ITS-DR2, which amplified a 567 bp band. The combination of COX-CF1 and COX-CR2 was the best set for discrimination of P. cryptogea/P. erythroseptica from other species which amplified a 415 bp product from both species. A restriction map analysis of P. cryptogea/P. erythroseptica indicated that the non-palindromic Mnl I enzyme restriction site was unique to amplicons of P. erythroseptica isolates and could be employed to distinguish this species from P. cryptogea. Based on this study, nested-PCR was at least 100 times more sensitive than conventional PCR for DETECTION of these species.

Cites

No record.

References

No record.

Cite

APA: Copy

MOSTOWFIZADEH GHALAMFARSA, R., & BANIHASHEMI, Z.. (2016). SPECIES-SPECIFIC PCR IDENTIFICATION AND DETECTION OF PHYTOPHTHORA DRECHSLERI, P. CRYPTOGEA AND P. ERYTHROSEPTICA. IRANIAN JOURNAL OF PLANT PATHOLOGY, 51(4), 541-553. SID. https://sid.ir/paper/48322/en

Vancouver: Copy

MOSTOWFIZADEH GHALAMFARSA R., BANIHASHEMI Z.. SPECIES-SPECIFIC PCR IDENTIFICATION AND DETECTION OF PHYTOPHTHORA DRECHSLERI, P. CRYPTOGEA AND P. ERYTHROSEPTICA. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2016;51(4):541-553. Available from: https://sid.ir/paper/48322/en

IEEE: Copy

R. MOSTOWFIZADEH GHALAMFARSA, and Z. BANIHASHEMI, “SPECIES-SPECIFIC PCR IDENTIFICATION AND DETECTION OF PHYTOPHTHORA DRECHSLERI, P. CRYPTOGEA AND P. ERYTHROSEPTICA,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 51, no. 4, pp. 541–553, 2016, [Online]. Available: https://sid.ir/paper/48322/en

Related Journal Papers