MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY AMONG IRANIAN POPULATIONS OF FUSARIUM GRAMINEARUM, THE CAUSAL AGENT OF WHEAT HEADBLIGHT

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SAFAEI NASER; ALIZADEH A.A.; SAEIDI ABAS; ADAM G.; RAHIMIAN H.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2005 | Volume:41 | Issue:2 Page(s): 171-189

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Title

MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY AMONG IRANIAN POPULATIONS OF FUSARIUM GRAMINEARUM, THE CAUSAL AGENT OF WHEAT HEADBLIGHT

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Keywords

GENETIC DIVERSITYQ4

SPECIES- SPECIFIC PRIMERSQ2

RAPD-PCRQ3

FUSARIUM GRAMINEARUMQ3

WHEAT FUSARIUM HEADBLIGHTQ4

IRANQ2

Abstract

Eighty-eight Fusarium isolates morphologically identified as FUSARIUM GRAMINEARUM and collected from northern, southern and northeastern parts of IRAN, were studied using species-specific (Schilling's) primers. The results indicated that a specific band of 332 bp was amplified, when species-specific primers of F. graminearum was used Of 88 isolates examined, 72 were molecularly identified as F. graminearum. The remaining 12 belonged to other species of Fusarium. The primers failed to amplify any band in negative controls including F. pseudograminearum and F. culmorum. To determine the extent of GENETIC DIVERSITY among IRANian populations of F. graminearum, eight random primers were tested for RAPD analysis. Of these, two primers (OPA07 and OPA10) were reproducibly polymorphic. The results of cluster analysis of OPA07 showed that at 85% similarity, isolates were categorized into IS groups. The 1st, 4th, 5th, 6th, 9th, 11th, groups contained 18, 4, 18, 20, 5 and 10% of isolates, respectively. The remaining groups had only one member. At 90% similarity, 29 groups were recognized. The cluster analysis for OPAIO categorized isolates into 9 groups at 85% similarity. The 1st, 2nd, 3rd, 6th and 7th contained 24, 26, 22, 14, 3 and 3% of isolates, respectively. Other groups had only one member. At 90% similarity, the isolates were divided into 19 groups. Using these two primers, the isolates from northwestern parts of IRAN were delineated from others. The isolates belonged to northern and southern parts of the country were distributed among various sub-clusters. This is the first report of molecular identification of F. graminearum using species-specific primers and the extent of its GENETIC DIVERSITY among IRANian populations of the species.

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APA: Copy

SAFAEI, NASER, ALIZADEH, A.A., SAEIDI, ABAS, ADAM, G., & RAHIMIAN, H.. (2005). MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY AMONG IRANIAN POPULATIONS OF FUSARIUM GRAMINEARUM, THE CAUSAL AGENT OF WHEAT HEADBLIGHT. IRANIAN JOURNAL OF PLANT PATHOLOGY, 41(2), 171-189. SID. https://sid.ir/paper/48346/en

Vancouver: Copy

SAFAEI NASER, ALIZADEH A.A., SAEIDI ABAS, ADAM G., RAHIMIAN H.. MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY AMONG IRANIAN POPULATIONS OF FUSARIUM GRAMINEARUM, THE CAUSAL AGENT OF WHEAT HEADBLIGHT. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2005;41(2):171-189. Available from: https://sid.ir/paper/48346/en

IEEE: Copy

NASER SAFAEI, A.A. ALIZADEH, ABAS SAEIDI, G. ADAM, and H. RAHIMIAN, “MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY AMONG IRANIAN POPULATIONS OF FUSARIUM GRAMINEARUM, THE CAUSAL AGENT OF WHEAT HEADBLIGHT,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 41, no. 2, pp. 171–189, 2005, [Online]. Available: https://sid.ir/paper/48346/en

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