INTRASPECIFIC ZYMOGRAPHY OF AG1-1A ISOLATES OF RHIZOCTONIA SOLANI, THE CAUSAL AGENT OF RICE SHEATH BLIGHT IN GOLESTAN PROVINCE

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KOWSARI M.; BAGHERI O.; BALALI G.R.; SALATI M.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2008 | Volume:44 | Issue:1 (173) Page(s): 22-36

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Title

INTRASPECIFIC ZYMOGRAPHY OF AG1-1A ISOLATES OF RHIZOCTONIA SOLANI, THE CAUSAL AGENT OF RICE SHEATH BLIGHT IN GOLESTAN PROVINCE

Author(s)

KOWSARI M. | BAGHERI O. | BALALI G.R. | SALATI M. | Issue Writer Certificate

Keywords

RICE SHEATH BLIGHTQ3

RHIZOCTONIA SOLANIQ3

PECTINASEQ3

ELECTROPHORESISQ3

Abstract

Sheath blight caused by RHIZOCTONIA SOLANI is one of the most important diseases of rice in Iran and almost in all rice-producing countries. R. solani AG1-IA is the causal agent of sheath blight in rice. It is believed that R. solani could use plant material by secretion of pectolytic enzyme such as PECTINASE. In this study samples were obtained during two years from diseased rice in from diseased rice in Golestan province of Iran. Seventy five isolates were identified as AG1-IA, based on the number of nuclei per cell and anastomosis reaction. The identified isolates of R. solani AG1-IA were used for pectic zymogram analyses. The pectic enzyme electrophoretic patterns were interpreted in terms of loci coding for polygalacturonase (PG) and pectic esterase (PE). Presence or absence of a band with a particular Rf value was scord as "1" and "0", respectively. The obtained zymogram electrophoretic patterns (ZPs) were divided into six groups: ZP1-ZP6. PG and PE bands were observed for all isolates tested. A total of 18 bands (15 PG and 3 PE) were identified, one PG and one PE appeared as cathodic bands. Inter-group relationships were deduced from the similarity matrices. The genetic similarity among zymogram electrophoretic phenotypes, ranged from 62% to 88% based on band frequency. The cluster analysis showed two main branches. ZP3 was clustered separately on a single branch, and the other branch was divided into two subbranches, one containing ZP5, and the other ZP1, ZP4. ZP2 and ZP6. The results indicated that there is a distinguishable diversity among different R. solani isolates of rice fields of Golestan province, and that could be detected by isozymes but not by anastomosis group. We concluded that the pectic enzyme ELECTROPHORESIS could be a suitable technique to study genetic variation of AG1-IA isolates.

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APA: Copy

KOWSARI, M., BAGHERI, O., BALALI, G.R., & SALATI, M.. (2008). INTRASPECIFIC ZYMOGRAPHY OF AG1-1A ISOLATES OF RHIZOCTONIA SOLANI, THE CAUSAL AGENT OF RICE SHEATH BLIGHT IN GOLESTAN PROVINCE. IRANIAN JOURNAL OF PLANT PATHOLOGY, 44(1 (173)), 22-36. SID. https://sid.ir/paper/48398/en

Vancouver: Copy

KOWSARI M., BAGHERI O., BALALI G.R., SALATI M.. INTRASPECIFIC ZYMOGRAPHY OF AG1-1A ISOLATES OF RHIZOCTONIA SOLANI, THE CAUSAL AGENT OF RICE SHEATH BLIGHT IN GOLESTAN PROVINCE. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2008;44(1 (173)):22-36. Available from: https://sid.ir/paper/48398/en

IEEE: Copy

M. KOWSARI, O. BAGHERI, G.R. BALALI, and M. SALATI, “INTRASPECIFIC ZYMOGRAPHY OF AG1-1A ISOLATES OF RHIZOCTONIA SOLANI, THE CAUSAL AGENT OF RICE SHEATH BLIGHT IN GOLESTAN PROVINCE,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 44, no. 1 (173), pp. 22–36, 2008, [Online]. Available: https://sid.ir/paper/48398/en

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