INDUCTION OF CYTOCHROME P4501A1 BY BETA-NAPHTHO FLAVONE AND DETERMINATION OF ENZYME PROPERTIES IN HUSO HUSO

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KARIMZADEH K.; MOSTAFAIE A.; ESMAELI A.; POURKAZEMI MOHAMMAD; ZAHMATKESH A.

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Journal: Iranian Scientific Fisheries Journal Year:2005 | Volume:14 | Issue:2 Page(s): 115-126

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Title

INDUCTION OF CYTOCHROME P4501A1 BY BETA-NAPHTHO FLAVONE AND DETERMINATION OF ENZYME PROPERTIES IN HUSO HUSO

Author(s)

Keywords

CYTOCHROME P4501A1Q4

BETA-NAPHTHO FLAVONEQ4

HUSO HUSOQ4

IRANQ3

Abstract

CYTOCHROME P4501A1 is a major isoenzyme in fish monooxygenase system which is induced by polycyclic aromatic hydrocarbons (PAHs) compounds. In this research, the inducing effect of p-naphtoflavone and its catalytic properties was studied in HUSO HUSO liver. Fish were given ip injection of p-naphthoflavone at three different doses. The enzyme activity was measured with de-ethylation of ethoxyresorufin reaction (ERGO) by flourometery method and relative amount of induced proteins were determined using polyacrylamid gel electrophoresis (SDS-PAGE).The results showed that EROD activity in the microsomal fraction of the treated fish was 15-26 folds that of the control group. Optimum activity of this enzyme was observed at 20-25 degrees centigrade. The maximum enzyme activity was seen in the precence of 180 micrograms of microsomal protein and 1.53µM of 7-ethoxyresorufin. SDS-PAGE of microsomal protein pattern in the treated fish revealed a protein with molecular mass 58±1 KDa translating to cytochrome P4501A. We conclude that the p-naphtoflovone in fish liver can induce cytochrome P450lA gene and increase its biosemhysis leading to raised enzyme activity in EROD reaction.

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APA: Copy

KARIMZADEH, K., MOSTAFAIE, A., ESMAELI, A., POURKAZEMI, MOHAMMAD, & ZAHMATKESH, A.. (2005). INDUCTION OF CYTOCHROME P4501A1 BY BETA-NAPHTHO FLAVONE AND DETERMINATION OF ENZYME PROPERTIES IN HUSO HUSO. IRANIAN SCIENTIFIC FISHERIES JOURNAL, 14(2), 115-126. SID. https://sid.ir/paper/5006/en

Vancouver: Copy

KARIMZADEH K., MOSTAFAIE A., ESMAELI A., POURKAZEMI MOHAMMAD, ZAHMATKESH A.. INDUCTION OF CYTOCHROME P4501A1 BY BETA-NAPHTHO FLAVONE AND DETERMINATION OF ENZYME PROPERTIES IN HUSO HUSO. IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2005;14(2):115-126. Available from: https://sid.ir/paper/5006/en

IEEE: Copy

K. KARIMZADEH, A. MOSTAFAIE, A. ESMAELI, MOHAMMAD POURKAZEMI, and A. ZAHMATKESH, “INDUCTION OF CYTOCHROME P4501A1 BY BETA-NAPHTHO FLAVONE AND DETERMINATION OF ENZYME PROPERTIES IN HUSO HUSO,” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 14, no. 2, pp. 115–126, 2005, [Online]. Available: https://sid.ir/paper/5006/en

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