MOLECULAR CLONING OF VP1 GENE OF FOOT-AND-MOUTH DISEASE VIRUS TYPE O1/IRAN

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MASOUDI S.; MOHAMMADI A.A.; MAHBOUDI F.; ROUSTAEI M.H.; KHEDMATI KAMBIZ

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Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2000 | Volume:- | Issue:51 Page(s): 1-14

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Title

MOLECULAR CLONING OF VP1 GENE OF FOOT-AND-MOUTH DISEASE VIRUS TYPE O1/IRAN

Author(s)

Keywords

CLONING

VP1 GENE

FMD VIRUS

ECHERISHIA COLI

Abstract

Double stranded cDNA coding for the immunogenic capsid protein VP1 of foot-and-mouth disease virus type O1/Iran from the virion RNA was prepared .Two primer pairs for VP1 GENE of virus were designed by comparison of published sequences and statistically analyzed using oligo and DNasis software. A PCR program was developed and optimized to amplify a segment of about 645 bp. Noninfected cells were used as negative control. The synthesized cDNA was then cloned into the NdeI and EcoR1 restriction enzymes site of pEt-23a+ plasmid using already attach linkers. The plasmid has been successfully propagated in and isolated from ECHERISHIA COLI bacterial culture and CLONING of VP1 GENE was confirmed by Dot Blot hybridization.

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APA: Copy

MASOUDI, S., MOHAMMADI, A.A., MAHBOUDI, F., ROUSTAEI, M.H., & KHEDMATI, KAMBIZ. (2000). MOLECULAR CLONING OF VP1 GENE OF FOOT-AND-MOUTH DISEASE VIRUS TYPE O1/IRAN. ARCHIVES OF RAZI INSTITUTE, -(51), 1-14. SID. https://sid.ir/paper/528064/en

Vancouver: Copy

MASOUDI S., MOHAMMADI A.A., MAHBOUDI F., ROUSTAEI M.H., KHEDMATI KAMBIZ. MOLECULAR CLONING OF VP1 GENE OF FOOT-AND-MOUTH DISEASE VIRUS TYPE O1/IRAN. ARCHIVES OF RAZI INSTITUTE[Internet]. 2000;-(51):1-14. Available from: https://sid.ir/paper/528064/en

IEEE: Copy

S. MASOUDI, A.A. MOHAMMADI, F. MAHBOUDI, M.H. ROUSTAEI, and KAMBIZ KHEDMATI, “MOLECULAR CLONING OF VP1 GENE OF FOOT-AND-MOUTH DISEASE VIRUS TYPE O1/IRAN,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 51, pp. 1–14, 2000, [Online]. Available: https://sid.ir/paper/528064/en

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