SOMATIC EMBRYOGENESISFROM ALFALFA (MEDICAGOSATIVA L.) CALLUS USING ETHINYLE ESTRADIOL

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EHSANPOUR A.A.; TAHERI R.

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Journal Paper

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Journal: Journal of Water and Soil Science Year:621 | Volume: | Issue: Page(s): 111-128

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Title

SOMATIC EMBRYOGENESISFROM ALFALFA (MEDICAGOSATIVA L.) CALLUS USING ETHINYLE ESTRADIOL

Author(s)

EHSANPOUR A.A. | TAHERI R. | Issue Writer Certificate

Keywords

ESTROGEN

ETHINYL ESTRADIOL

ALFALFA

PLANT REGENERATION.

Abstract

In this research the effect of ETHINYL ESTRADIOL on somatic embryogenesis of Medicago sativa was investigated. Seeds were grown in vitro on MS medium containing 2,4-D, Kin and NAA for callus induction. PLANT REGENERATION.media with 14 different combinations of auxin, cytokinin and ETHINYL ESTRADIOL were provided. Three to four-week old celli and stem or hypocotyl segments of Medicago sativa were transferred to regeneration media. Results showed that ETHINYL ESTRADIOL (I mg/L) induced shoot, root and somatic embryo on calli. When the stem and hypocotyl segments were cultured on medium M12, only shoots and roots were produced with no somatic embryo. Finally, it was observed that ethinyl stradiol and auxin together could induce somatic embryogenesis and plant regeneration, while these hormones had no effect separately.

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APA: Copy

EHSANPOUR, A.A., & TAHERI, R.. (2005). SOMATIC EMBRYOGENESISFROM ALFALFA (MEDICAGOSATIVA L.) CALLUS USING ETHINYLE ESTRADIOL. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), 9(1), 111-128. SID. https://sid.ir/paper/528715/en

Vancouver: Copy

EHSANPOUR A.A., TAHERI R.. SOMATIC EMBRYOGENESISFROM ALFALFA (MEDICAGOSATIVA L.) CALLUS USING ETHINYLE ESTRADIOL. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES)[Internet]. 2005;9(1):111-128. Available from: https://sid.ir/paper/528715/en

IEEE: Copy

A.A. EHSANPOUR, and R. TAHERI, “SOMATIC EMBRYOGENESISFROM ALFALFA (MEDICAGOSATIVA L.) CALLUS USING ETHINYLE ESTRADIOL,” WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), vol. 9, no. 1, pp. 111–128, 2005, [Online]. Available: https://sid.ir/paper/528715/en

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