CLONING OF BINDING DOMAIN OF CLOSTRIDIUM BOTULINUM TOXIN TYPE A IN ESCHERICHIA COLI

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TAVALLAIE M.; SALMANIAN A.H.; BOUZARI S.

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Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2000 | Volume:- | Issue:51 Page(s): 113-121

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Information Journal Paper

Title

CLONING OF BINDING DOMAIN OF CLOSTRIDIUM BOTULINUM TOXIN TYPE A IN ESCHERICHIA COLI

Author(s)

TAVALLAIE M. | SALMANIAN A.H. | BOUZARI S. | Issue Writer Certificate

Keywords

BOTULINUM

NEUROTOXIN

BINDING DOMAIN

EXPRESSION

ESCHERICHIA COLI

Abstract

Neurotoxin of Clostridium BOTULINUM is one of the most potent known toxins in nature, which can cause an often fatal food poisoning. However, recently the use of BINDING DOMAIN of the toxin has also been proposed as a candidate vaccine. In this study coding region for this domain was amplified from genomic DNA of Clostridium BOTULINUM type A using a pair of primers containing BamHI and HindIII. The amplified l.3kb fragment corresponding to the gene of interest was cloned in pUC18 after verification with different restriction enzymes. The construct was then transformed into competent ESCHERICHIA COLI DH5α.and the recombinant clone was selected on plates containing ampicillin.The plasmid purified from these clones was used for sequencing by dideoxy chain termination method. The sequence thus obtained was verified by comparison with published sequence, which showed the authencity of the PCR product. This fragment was subcloned in an IPTG inducible EXPRESSION vector pQE30 using E.coli M15 as a host and EXPRESSION was analyzed by RT-PCR.

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APA: Copy

TAVALLAIE, M., SALMANIAN, A.H., & BOUZARI, S.. (2000). CLONING OF BINDING DOMAIN OF CLOSTRIDIUM BOTULINUM TOXIN TYPE A IN ESCHERICHIA COLI. ARCHIVES OF RAZI INSTITUTE, -(51), 113-121. SID. https://sid.ir/paper/529337/en

Vancouver: Copy

TAVALLAIE M., SALMANIAN A.H., BOUZARI S.. CLONING OF BINDING DOMAIN OF CLOSTRIDIUM BOTULINUM TOXIN TYPE A IN ESCHERICHIA COLI. ARCHIVES OF RAZI INSTITUTE[Internet]. 2000;-(51):113-121. Available from: https://sid.ir/paper/529337/en

IEEE: Copy

M. TAVALLAIE, A.H. SALMANIAN, and S. BOUZARI, “CLONING OF BINDING DOMAIN OF CLOSTRIDIUM BOTULINUM TOXIN TYPE A IN ESCHERICHIA COLI,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 51, pp. 113–121, 2000, [Online]. Available: https://sid.ir/paper/529337/en

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