POLYMERASE CHAIN REACTION FOR THE DETECTION AND DIFFERENTIATION OF MAREK’S DISEASE VIRUS STRAINS MDV-1 AND HVT

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SADEGHI M.R.; MORSHEDI D.; SALEHI TABAR R.; GHOREYSHI S.A.; KARGAR MOAKHAR R.; GHAEM MAGHAMI S.S.

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Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2006 | Volume:7 | Issue:1 (14) Page(s): 0-0

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Title

POLYMERASE CHAIN REACTION FOR THE DETECTION AND DIFFERENTIATION OF MAREK’S DISEASE VIRUS STRAINS MDV-1 AND HVT

Author(s)

Keywords

MAREK’S DISEASE VIRUS

PCR

DETECTION

Abstract

Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocytic infiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) to optimize a rapid and reliable assay for DETECTION of MDV genome. DETECTION of serotype 1 of MDV (MDV-1) was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 and herpesvirus of turkeys (HVT) was also conducted using specific primers from the glycoprotein A (gA) gene and a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed by sequencing of PCR products. Results indicate that MDV-1 can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV-1 and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and can be performed as a robust diagnostic test in veterinary diagnostic laboratories

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APA: Copy

SADEGHI, M.R., MORSHEDI, D., SALEHI TABAR, R., GHOREYSHI, S.A., KARGAR MOAKHAR, R., & GHAEM MAGHAMI, S.S.. (2006). POLYMERASE CHAIN REACTION FOR THE DETECTION AND DIFFERENTIATION OF MAREK’S DISEASE VIRUS STRAINS MDV-1 AND HVT. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 7(1 (14)), 0-0. SID. https://sid.ir/paper/529485/en

Vancouver: Copy

SADEGHI M.R., MORSHEDI D., SALEHI TABAR R., GHOREYSHI S.A., KARGAR MOAKHAR R., GHAEM MAGHAMI S.S.. POLYMERASE CHAIN REACTION FOR THE DETECTION AND DIFFERENTIATION OF MAREK’S DISEASE VIRUS STRAINS MDV-1 AND HVT. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2006;7(1 (14)):0-0. Available from: https://sid.ir/paper/529485/en

IEEE: Copy

M.R. SADEGHI, D. MORSHEDI, R. SALEHI TABAR, S.A. GHOREYSHI, R. KARGAR MOAKHAR, and S.S. GHAEM MAGHAMI, “POLYMERASE CHAIN REACTION FOR THE DETECTION AND DIFFERENTIATION OF MAREK’S DISEASE VIRUS STRAINS MDV-1 AND HVT,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 7, no. 1 (14), pp. 0–0, 2006, [Online]. Available: https://sid.ir/paper/529485/en

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