STANDARDIZATION OF ANTI-DNA ELISA KIT IN RAZI INSTITUTE

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MOUSAVI T.; AKHAVIZADEGAN M.A.; ASADI N.

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Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2003 | Volume:- | Issue:55 Page(s): 55-62

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Information Journal Paper

Title

STANDARDIZATION OF ANTI-DNA ELISA KIT IN RAZI INSTITUTE

Author(s)

MOUSAVI T. | AKHAVIZADEGAN M.A. | ASADI N. | Issue Writer Certificate

Keywords

ELISA

ANTI-DNA ANTIBODY

SLE

Abstract

In order to develop an ELISA kit for detection of anti-DNA antibodies several procedures were examined. In this study the best method for native DNA preparation, solid phase preparation, and formulation of buffers, design of test performance, and a storage condition of the kit components was introduced. DNA extraction was performed by phenol procedure and purified one more time to lose protein impurities. To prepare the coated micro plate, a percoating stage using poly-L lysine to enhance DNA attachment and an extra post-coating stage for neutralizing its negative charge were applied. To determine the sensitivity and specificity, 120 serum samples were simultaneously tested with the developed and commercial kits. The results indicate that sensitivity and specificity of the developed kit is 97% and 100% respectively with 99% accuracy and 5% coefficient of variations. Moreover periodic examinations on kit components reveal that the kit is stable at least one year in 4°C without diminished quality.

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APA: Copy

MOUSAVI, T., AKHAVIZADEGAN, M.A., & ASADI, N.. (2003). STANDARDIZATION OF ANTI-DNA ELISA KIT IN RAZI INSTITUTE. ARCHIVES OF RAZI INSTITUTE, -(55), 55-62. SID. https://sid.ir/paper/530609/en

Vancouver: Copy

MOUSAVI T., AKHAVIZADEGAN M.A., ASADI N.. STANDARDIZATION OF ANTI-DNA ELISA KIT IN RAZI INSTITUTE. ARCHIVES OF RAZI INSTITUTE[Internet]. 2003;-(55):55-62. Available from: https://sid.ir/paper/530609/en

IEEE: Copy

T. MOUSAVI, M.A. AKHAVIZADEGAN, and N. ASADI, “STANDARDIZATION OF ANTI-DNA ELISA KIT IN RAZI INSTITUTE,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 55, pp. 55–62, 2003, [Online]. Available: https://sid.ir/paper/530609/en

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