IMMUNOGENICITY AND EFFICACY OF BACULOVIRUS DERIVED GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS TYPE-1 IN MICE

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SOLEYMANJAHI H.; ROUSTAEI M.H.; RASSAEE M.J.; MAHBOUDI F.; BAMDAD T.

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Journal Paper

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2003 | Volume:- | Issue:55 Page(s): 19-28

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Title

IMMUNOGENICITY AND EFFICACY OF BACULOVIRUS DERIVED GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS TYPE-1 IN MICE

Author(s)

Keywords

HERPES SIMPLEX VIRUS GD

RECOMBINANT PROTEIN

WESTERN BLOTTING

IMMUNIZATION

Abstract

The recombinant glycoprotein D (gD) of herpes simplex virus type- 1 (HSV-1) in baculovirus expression system was produced. Spodoptera Frugiperda cell, clone 9 (Sf9) was cultured in modified Graces medium and inoculated with 57 multiplicity of infection of HSV-1 recombinant baculovirus carrying gD gene. Inoculated cells were harvested 96h postinoculation and treated with 3[(3-cholamidopropy1)dimethylainmonio]-1-propanesulfonate (CHAPS) and phenylmethylsulfonyl fluoride (PMSF) and sonicated at 20KHZ for 5 times. A WESTERN BLOTTING NNas developed and applied to detect the prepared protein. Three groups of BALB/c mice each of 7 mice were inoculated with the recombinant gD, Sublethal dose of challenge virus (104.5 TCID50) and PBS respectively. All inoculated mice were challenged with 10MLD50 (106.5 TCID50) of the wild HSV-1. All mice who had received the recombinant gD survived while 14.3% and 71.5% of mice inoculated respectively with either Sublethal dose of the virus or PBS died.

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APA: Copy

SOLEYMANJAHI, H., ROUSTAEI, M.H., RASSAEE, M.J., MAHBOUDI, F., & BAMDAD, T.. (2003). IMMUNOGENICITY AND EFFICACY OF BACULOVIRUS DERIVED GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS TYPE-1 IN MICE. ARCHIVES OF RAZI INSTITUTE, -(55), 19-28. SID. https://sid.ir/paper/530836/en

Vancouver: Copy

SOLEYMANJAHI H., ROUSTAEI M.H., RASSAEE M.J., MAHBOUDI F., BAMDAD T.. IMMUNOGENICITY AND EFFICACY OF BACULOVIRUS DERIVED GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS TYPE-1 IN MICE. ARCHIVES OF RAZI INSTITUTE[Internet]. 2003;-(55):19-28. Available from: https://sid.ir/paper/530836/en

IEEE: Copy

H. SOLEYMANJAHI, M.H. ROUSTAEI, M.J. RASSAEE, F. MAHBOUDI, and T. BAMDAD, “IMMUNOGENICITY AND EFFICACY OF BACULOVIRUS DERIVED GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS TYPE-1 IN MICE,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 55, pp. 19–28, 2003, [Online]. Available: https://sid.ir/paper/530836/en

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