DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN ALLANTIOC FLUID BY RAPID HEMAGGLUTINATION TEST

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MOMAYEZ R.; TOROUGHI R.; POURBAKHSH S.A.; GHARAH KHANI P.

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Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2005 | Volume:- | Issue:59 Page(s): 47-54

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Information Journal Paper

Title

DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN ALLANTIOC FLUID BY RAPID HEMAGGLUTINATION TEST

Author(s)

MOMAYEZ R. | TOROUGHI R. | POURBAKHSH S.A. | GHARAH KHANI P. | Issue Writer Certificate

Keywords

INFECTIOUS BRONCHITIS VIRUS

ALLANTOIC FLUID

RAPID HEMAGGLUTINATION

NEURAMINIDASE ENZYME

RT-PCR

Abstract

To detect the presence of INFECTIOUS BRONCHITIS VIRUS (IBV) in infected ALLANTOIC FLUID (AF) of SPF embryonated eggs RAPID HEMAGGLUTINATION (HA) activity after treatment with NEURAMINIDASE ENZYME was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harveste AFs were treated with NEURAMINIDASE ENZYME and the presence of IBV was detected by inducing hemagglutination of chicken red blood cells. The specificity of rapid HA test was examined with three non-hemagglutinating avian viruses including infectious bursal disease virus, infectious laryngotracheitis virus and fowl pox virus. The sensitivity of the test was compared with reversed transcriptase-polymerase chain reaction. The results showed that this test was specific and had a sensitivity of 100% for IBV detection. The results of this study indicate that HA test using neuraminidase treatment is an accurate, sensitive, specific and inexpensive test for rapid detection of IBV.

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APA: Copy

MOMAYEZ, R., TOROUGHI, R., POURBAKHSH, S.A., & GHARAH KHANI, P.. (2005). DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN ALLANTIOC FLUID BY RAPID HEMAGGLUTINATION TEST. ARCHIVES OF RAZI INSTITUTE, -(59), 47-54. SID. https://sid.ir/paper/538294/en

Vancouver: Copy

MOMAYEZ R., TOROUGHI R., POURBAKHSH S.A., GHARAH KHANI P.. DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN ALLANTIOC FLUID BY RAPID HEMAGGLUTINATION TEST. ARCHIVES OF RAZI INSTITUTE[Internet]. 2005;-(59):47-54. Available from: https://sid.ir/paper/538294/en

IEEE: Copy

R. MOMAYEZ, R. TOROUGHI, S.A. POURBAKHSH, and P. GHARAH KHANI, “DETECTION OF INFECTIOUS BRONCHITIS VIRUS IN ALLANTIOC FLUID BY RAPID HEMAGGLUTINATION TEST,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 59, pp. 47–54, 2005, [Online]. Available: https://sid.ir/paper/538294/en

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