مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

PHENOTYPIC CHARACTERIZATION AND PCR-RIBOTYPING OF PSEUDOMONAS FLUORESCENS ISOLATES, IN TRACKING CONTAMINATION ROUTES IN THE PRODUCTION LINE OF PASTEURIZED MILK

Pages

  222-232

Abstract

 Contamination sites of PSEUDOMONAS FLUORESCENS were traced in the production line for MILK pasteurization in a large dairy plant in Shiraz, Iran. Samples of raw and pasteurized MILK were collected at six sites along the line. All MILK samples were incubated at 7°C until the aerobic plate count had reached 106-107 cfu mL-1. Colonies were picked randomly and identified. No growth of gram negative psychrotrophic bacteria (GNP) was detected in the immediately pasteurized MILK samples (just after the pasteurization), during long incubation at 7°C. RECONTAMINATION most often occurred in the filling step. In this study 34.1 and 4.9% of the MILK packages showed contamination with GNP and P. fluorescens, respectively. Twenty three P. fluorescens isolates were examined for phenotypic characteristics and 16S-23S PCR ribotyping. Phylogenetic analysis was conducted on the phenotypic and genotypic characteristics. The P. fluorescens isolates were shown to belong to 6 biotypes (B1-B6). The predominance of a particular ribotype was often observed for a given biotype, although there were two ribotypes in each of the B2 and B6 biotypes. The 16S-23S PCR-RIBOTYPING technique allowed differentiation between the isolates. Based on this method, the isolates belonged to 5 subtypes. Phylogenetic analysis based on 16S-23S PCR-RIBOTYPING and phenotypic characterization could be helpful in tracking contamination routes in the production line for MILK pasteurization.

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    • Cite

    APA: Copy

    BEDEL TAVANA, A., HAGHKHAH, M., & NAZER, A.. (2010). PHENOTYPIC CHARACTERIZATION AND PCR-RIBOTYPING OF PSEUDOMONAS FLUORESCENS ISOLATES, IN TRACKING CONTAMINATION ROUTES IN THE PRODUCTION LINE OF PASTEURIZED MILK. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 11(3 (32)), 222-232. SID. https://sid.ir/paper/556819/en

    Vancouver: Copy

    BEDEL TAVANA A., HAGHKHAH M., NAZER A.. PHENOTYPIC CHARACTERIZATION AND PCR-RIBOTYPING OF PSEUDOMONAS FLUORESCENS ISOLATES, IN TRACKING CONTAMINATION ROUTES IN THE PRODUCTION LINE OF PASTEURIZED MILK. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2010;11(3 (32)):222-232. Available from: https://sid.ir/paper/556819/en

    IEEE: Copy

    A. BEDEL TAVANA, M. HAGHKHAH, and A. NAZER, “PHENOTYPIC CHARACTERIZATION AND PCR-RIBOTYPING OF PSEUDOMONAS FLUORESCENS ISOLATES, IN TRACKING CONTAMINATION ROUTES IN THE PRODUCTION LINE OF PASTEURIZED MILK,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 11, no. 3 (32), pp. 222–232, 2010, [Online]. Available: https://sid.ir/paper/556819/en

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