ASSESSMENT OF GENETIC DIVERSITY IN ALFALFA (MEDICAGO SATIVA L.) ECOTYPES FROM CENTRAL AND EASTERN REGIONS OF IRAN USING SSR MARKERS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

REZAIE M.; NAGHAVI M.R.; MAALI AMIRI R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Crop Sciences Year:2011 | Volume:12 | Issue:4 (48) Page(s): 520-532

Download Full-Text

Persian Verion

View:

842

Download:

Cites:

Information Journal Paper

Title

ASSESSMENT OF GENETIC DIVERSITY IN ALFALFA (MEDICAGO SATIVA L.) ECOTYPES FROM CENTRAL AND EASTERN REGIONS OF IRAN USING SSR MARKERS

Author(s)

REZAIE M. | NAGHAVI M.R. | MAALI AMIRI R. | Issue Writer Certificate

Keywords

ALFALFAQ2

GENETIC VARIATION AND MICROSATELLITE MARKERQ3

Abstract

Genetic variation in 33 ALFALFA ecotypes from central and eastern regions of Iran was assessed using SSR markers. PCR analysis was performed on genomic DNA using 10 microsatellite primer pairs. PCR products were detected using denatured polyacrylamide gel. From 62 reproducible bands identified, 52 bands were polymorphic corresponding to an average of 5 bands per locus. The proportion of polymorphism with an average of 80.64% varied from 62.5 for MTIC250 to 100 for ENOD 20. The average of polymorphism information content (PIC) was 0.61. ENOD20 and MAL369471 primers had the highest PIC value (0.78 and 0.83) and the lowest one (0.37 and 0.34) in central and eastern ecotypes, respectively. Nei’s gene diversity and Shannon’s information index for both of populations were calculated and their values were 0.29, 0.3 and 0.45, 0.46, respectively. Genetic similarity was estimated using the Dice coefficient, and dendrogram was constructed using the UPGMA method. Generally, results showed that despite of the similarities of genetic structures between the ecotypes from two regions, high variation was observed among individual plants possibly due to the high allogamy (90%), insects activity (particularly bees) and pollen transmission among ecotypes. Genetic similarity between ecotypes in this study could be considered as consequeneces of genetic equilibrium that have been occurred over long time of cultivation of ALFALFA in these regions as well as seed exchange among provinces.

Cites

No record.

References

No record.

Cite

APA: Copy

REZAIE, M., NAGHAVI, M.R., & MAALI AMIRI, R.. (2011). ASSESSMENT OF GENETIC DIVERSITY IN ALFALFA (MEDICAGO SATIVA L.) ECOTYPES FROM CENTRAL AND EASTERN REGIONS OF IRAN USING SSR MARKERS. IRANIAN JOURNAL OF CROP SCIENCES, 12(4 (48)), 520-532. SID. https://sid.ir/paper/57319/en

Vancouver: Copy

REZAIE M., NAGHAVI M.R., MAALI AMIRI R.. ASSESSMENT OF GENETIC DIVERSITY IN ALFALFA (MEDICAGO SATIVA L.) ECOTYPES FROM CENTRAL AND EASTERN REGIONS OF IRAN USING SSR MARKERS. IRANIAN JOURNAL OF CROP SCIENCES[Internet]. 2011;12(4 (48)):520-532. Available from: https://sid.ir/paper/57319/en

IEEE: Copy

M. REZAIE, M.R. NAGHAVI, and R. MAALI AMIRI, “ASSESSMENT OF GENETIC DIVERSITY IN ALFALFA (MEDICAGO SATIVA L.) ECOTYPES FROM CENTRAL AND EASTERN REGIONS OF IRAN USING SSR MARKERS,” IRANIAN JOURNAL OF CROP SCIENCES, vol. 12, no. 4 (48), pp. 520–532, 2011, [Online]. Available: https://sid.ir/paper/57319/en

Related Journal Papers