SOMATIC EMBRYOGENESIS IN THREE COTTON (GOSSYPIUM HIRSUTUM L.) CULTIVARS

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GAROUSI SH.; TOUHIDFAR M.; KAZEMI TABAR S.K.; RAHIMIAN H.A.; NEMATZADEH GH.A.

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Journal Paper

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Journal: Iranian Journal of Crop Sciences Year:2008 | Volume:9 | Issue:4 (36) Page(s): 302-314

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Title

SOMATIC EMBRYOGENESIS IN THREE COTTON (GOSSYPIUM HIRSUTUM L.) CULTIVARS

Author(s)

Keywords

COTTONQ3

REGENERATIONQ2

SOMATIC EMBRYOGENESISQ3

TISSUE CULTUREQ3

Abstract

In COTTON (Gossypium hirsutum L.) transformation, for regenerating plants from a single cell, an optimized TISSUE CULTURE system is necessary. Successful production of transgenic COTTON depends on regenerating of many plants; therefore, SOMATIC EMBRYOGENESIS was investigated for three COTTON cultivars. Hypocotyls of cvs. Sahel, Varamin and Coker 312 were isolated and placed on two COTTON callus induction media containing MSB medium supplemented with 0.1 mg/l 2,4-D, 0.1 mg/l kinetin, 0.75 g/l MgCl2; and MS medium supplemented with 0.1 mg/l Kinetin and 0.2 mg/l IBA. In medium containing 2,4-D and Kinetin, Sahel and Coker312 explants produced callus earlier than Varamin and the volume of calli were larger, too. The percentage of callus production for Sahel cultivar was higher than Varamin. For embryogenesis induction and maturation of embryos, produced calli were transferred onto MSB medium supplemented with 0.75 g/l MgCl2 and 1.9 g/l KNo3. Numbers of embryogenic calli for Coker312 were higher than Sahel. Germination of somatic embryos for Coker312 and Sahel on embryo germination medium containing MSB supplemented with 0.1 mg/l Zeatin and 2 g/l activated charcoal were earlier than Varamin. Percentage of somatic embryos produced from Coker 312 was higher than Sahel. For rooting, there were no significant differences among COTTON cultivars and five rooting media.

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References

ASSESSMENT OF DIRECT REGENERATION POTENTIAL OF THREE COTTON CULTIVARS THROUGH SHOOT APEX CULTURE

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APA: Copy

GAROUSI, SH., TOUHIDFAR, M., KAZEMI TABAR, S.K., RAHIMIAN, H.A., & NEMATZADEH, GH.A.. (2008). SOMATIC EMBRYOGENESIS IN THREE COTTON (GOSSYPIUM HIRSUTUM L.) CULTIVARS. IRANIAN JOURNAL OF CROP SCIENCES, 9(4 (36)), 302-314. SID. https://sid.ir/paper/57338/en

Vancouver: Copy

GAROUSI SH., TOUHIDFAR M., KAZEMI TABAR S.K., RAHIMIAN H.A., NEMATZADEH GH.A.. SOMATIC EMBRYOGENESIS IN THREE COTTON (GOSSYPIUM HIRSUTUM L.) CULTIVARS. IRANIAN JOURNAL OF CROP SCIENCES[Internet]. 2008;9(4 (36)):302-314. Available from: https://sid.ir/paper/57338/en

IEEE: Copy

SH. GAROUSI, M. TOUHIDFAR, S.K. KAZEMI TABAR, H.A. RAHIMIAN, and GH.A. NEMATZADEH, “SOMATIC EMBRYOGENESIS IN THREE COTTON (GOSSYPIUM HIRSUTUM L.) CULTIVARS,” IRANIAN JOURNAL OF CROP SCIENCES, vol. 9, no. 4 (36), pp. 302–314, 2008, [Online]. Available: https://sid.ir/paper/57338/en

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