Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

381
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

174
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

THE EFFECT OF CITRIC ACID AND CITRATE ON PROTOPLASMIC DROPLET OF BOVINE EPIDIDYMAL SPERMS

Pages

  260-264

Abstract

 For evaluation of CITRIC ACID and citrate effects on bovine epididymal protoplasmic droplets, fifty bovine testes were collected in the October 2007 till June 2008 from Urmia slaughterhouse and transported to the laboratory in a cool container filled with 5oC ice pack. Caudal epididymis was incised and sperm cells were put into Petri dishes containing hams f10 media with 10% fetal calf serum (FCS), which were kept in 37oC, CO2 incubator. Then sperm cells were counted and 50-milion per mL concentration was prepared. After this stage, three dilutions of CITRIC ACID (0.1, 0.2, 0.3 N) and one dilution of citrate (1 N), based on normal osmolarity and normal pH were added to a micro tube containing 25 milion per mL sperm. Then one-step eosin-nigrosin staining in 30-60-120-240-360 minutes was performed and slides were evaluated with 1000-x phase contrast microscope and 200 sperm cells per slide were counted. The results revealed significant difference between blank and CITRIC ACID 0.3 N. The proportion of protoplasmic droplet in group consisting of 0.3 N acid citric in 120-240-360 minutes, was significantly lower than that of blank (P<0.05). There was no significant difference between citrate – blank and CITRIC ACID 0.1 N-blank groups, but after 240 minutes significant difference was observed between blank & CITRIC ACID 0.2 N (P<0.05). In conclusions CITRIC ACID based on dilution and time duration can reduce the proportion of bovine EPIDIDYMAL SPERM cytoplasmic droplets.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    ABDY, KEIVAN, & TAJIK, PARVIZ. (2011). THE EFFECT OF CITRIC ACID AND CITRATE ON PROTOPLASMIC DROPLET OF BOVINE EPIDIDYMAL SPERMS. VETERINARY RESEARCH FORUM, 2(4), 260-264. SID. https://sid.ir/paper/591749/en

    Vancouver: Copy

    ABDY KEIVAN, TAJIK PARVIZ. THE EFFECT OF CITRIC ACID AND CITRATE ON PROTOPLASMIC DROPLET OF BOVINE EPIDIDYMAL SPERMS. VETERINARY RESEARCH FORUM[Internet]. 2011;2(4):260-264. Available from: https://sid.ir/paper/591749/en

    IEEE: Copy

    KEIVAN ABDY, and PARVIZ TAJIK, “THE EFFECT OF CITRIC ACID AND CITRATE ON PROTOPLASMIC DROPLET OF BOVINE EPIDIDYMAL SPERMS,” VETERINARY RESEARCH FORUM, vol. 2, no. 4, pp. 260–264, 2011, [Online]. Available: https://sid.ir/paper/591749/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops

  • No record.





    • Move to top