DETECTION OF MONOSPORASCUS CANNONBALLUS FROM MELON PLANTS USING PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SARPELEH ABOLFAZL; CHERAGHALI VADIEH; RAZAVI MOHAMMAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CROP PROTECTION Year:2012 | Volume:1 | Issue:4 Page(s): 349-359

Download Full-Text

View:

371

Download:

178

Cites:

Information Journal Paper

Title

DETECTION OF MONOSPORASCUS CANNONBALLUS FROM MELON PLANTS USING PCR

Author(s)

SARPELEH ABOLFAZL | CHERAGHALI VADIEH | RAZAVI MOHAMMAD | Issue Writer Certificate

Keywords

MELON

MONOSPORASCUS CANNONBALLUS

PCR

Abstract

Root rot and vine decline caused by MONOSPORASCUS CANNONBALLUS is a major challenge for MELON production world-wide. In recent years, a disease suggested to be related to this pathogen was observed 1-2 weeks prior to harvest in many MELON production areas across Iran. In this study, MELON plants with symptoms of chlorosis, wilting, decline and/or sudden death were collected from MELON growing areas. Pieces of the roots with rot symptoms or discoloration were surface-sterilized and placed on PDA culture medium.DNA was extracted from the rest of the sterilized roots and used in polymerase chain reaction (PCR) using specific primers designed from ribosomal DNA of M. cannonballus. The pathogenicity of the fungus for 24 of its isolates was examined on a muskMELON genotype, Zard-e-Garmsar. In addition, the presence of M. cannonballus was tested on the symptomless MELON plants at early growing stages as well as those inoculated with this pathogen using the specific primers. The presence of M. cannonballus was confirmed in 95 MELON samples (63% of total samples tested) based on the morphological criteria of the isolated fungus and molecular techniques, where a unique band specific to this pathogen was amplified in diagnostic PCR.M. cannonballus was also detected in the roots of symptomless and inoculated MELON plants as early as 2 days post-inoculation. This study demonstrated that M. cannonballus is the major causal organism for MELON collapse in all sampling regions and that the pathogen is detectable in MELON plants suspected of infection using molecular tools at early growth stages.

Cites

FUNCTIONAL SECRETOMICS OF PHYTOTOXIC COMPOUNDS OF MONOSPORASCUS CANNONBALLUS

References

No record.

Cite

APA: Copy

SARPELEH, ABOLFAZL, CHERAGHALI, VADIEH, & RAZAVI, MOHAMMAD. (2012). DETECTION OF MONOSPORASCUS CANNONBALLUS FROM MELON PLANTS USING PCR. JOURNAL OF CROP PROTECTION, 1(4), 349-359. SID. https://sid.ir/paper/597546/en

Vancouver: Copy

SARPELEH ABOLFAZL, CHERAGHALI VADIEH, RAZAVI MOHAMMAD. DETECTION OF MONOSPORASCUS CANNONBALLUS FROM MELON PLANTS USING PCR. JOURNAL OF CROP PROTECTION[Internet]. 2012;1(4):349-359. Available from: https://sid.ir/paper/597546/en

IEEE: Copy

ABOLFAZL SARPELEH, VADIEH CHERAGHALI, and MOHAMMAD RAZAVI, “DETECTION OF MONOSPORASCUS CANNONBALLUS FROM MELON PLANTS USING PCR,” JOURNAL OF CROP PROTECTION, vol. 1, no. 4, pp. 349–359, 2012, [Online]. Available: https://sid.ir/paper/597546/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops